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Full Discussion: Help with a bash loop script
Top Forums UNIX for Beginners Questions & Answers Help with a bash loop script Post 303037350 by dre on Tuesday 30th of July 2019 09:34:25 AM
Old 07-30-2019
Help with a bash loop script

Create a single bash script that does the following:
a. Print out the number of occurrences for each motif that is found in the bacterial genome and output to a file called motif_count.txt
b. Create a fasta file for each motif (so 3 in total) which contains all of the genes and their corresponding sequences that have that motif. Each file should be named after the motif (ie ATG.txt) and outputted to a new directory called motifs

motif file is a txt file with these motifs : ATG, GGGGG, ATTTT
the bacterial genome file is fasta file with the following lines
Code:
 >gene1
GAAACTCGGTGTTGGCTTACCGGTCATTCCGAGCGTCATTTGGTTTTCGCGTCGTGGCGAAATGTGGTTCTACTACTCGTGGTGTATGCACTATTTATCCGGAATGTTCAGAGCGAGTAGACAATGGGTGCTCCACAATTGTGGCGGTCCCTAAGGGACTCACATATAGTGAGACACGCGTGAAATTCTGCTCACCACGTCCGAATCCGACAAATCATCTACTTCGACGGTA
>gene2
CGGAGATAAAGGACCCATACTGTACGACATTGTATTGCTCACCATGGTCAATCTTTGCGAGTTGTTGCAGCTCGCAGCTTCGTTCTGTCAATATAGCTTAGATACTGAGAAGAAGTTGCAGAGAAAGTCGCA

Moderator's Comments:
Mod Comment edit by bakunin: please use CODE-tags to let data, code and terminal output stand out. Thank you.

Last edited by bakunin; 07-30-2019 at 10:48 AM..
 

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GLAM2SCAN(1)							   glam2 Manual 						      GLAM2SCAN(1)

NAME
glam2scan - finds a GLAM2 motif in a database SYNOPSIS
glam2scan [options] alphabet my_motif.glam2 my_seqs.fa An alphabet other than p or n is interpreted as the name of an alphabet file. DESCRIPTION
glam2scan finds matches, in a sequence database, to a motif discovered by glam2. Each match receives a score, indicating how well it fits the motif. OPTIONS (DEFAULT SETTINGS) -h Show all options and their default settings. -o Output file (stdout). -n Number of alignments to report (25). -2 Examine both strands - forward and reverse complement. -D Deletion pseudocount (0.1). -E No-deletion pseudocount (2.0). -I Insertion pseudocount (0.02). -J No-insertion pseudocount (1.0). -d Dirichlet mixture file. SEE ALSO
glam2format(1), glam2mask(1), glam2-purge(1), glam2(1) The full Hypertext documentation of GLAM2 is available online at http://bioinformatics.org.au/glam2/ or on this computer in /usr/share/doc/glam2/. REFERENCE
If you use GLAM2, please cite: MC Frith, NFW Saunders, B Kobe, TL Bailey (2008) Discovering sequence motifs with arbitrary insertions and deletions, PLoS Computational Biology (in press). AUTHORS
Martin Frith Author of GLAM2. Timothy Bailey Author of GLAM2. Charles Plessy <plessy@debian.org> Formatted this manpage in DocBook XML for the Debian distribution. COPYRIGHT
The source code and the documentation of GLAM2 are released in the public domain. GLAM2 1056 05/19/2008 GLAM2SCAN(1)
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