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NTDPAL(1)						       Primer3 User Manuals							 NTDPAL(1)

NAME
ntdpal - Provides Primer3's alignment functionality SYNOPSIS
ntdpal [-g gval] [-l lval] [-m mval] [-f1, f2, f3] [-p] [-s] [-e] {seq1} {seq2} {mode} DESCRIPTION
Ntdpal (NucleoTide Dynamic Programming ALignment) is a stand-alone program that provides Primer3's alignment functionality (local, a.k.a. Smith-Waterman, global, a.k.a. Needleman-Wunsch, plus "half global"). OPTIONS
-g gval gval is a (positive) float (.01 precision) specifying penaltiy for creating a gap respectively (the penalties are subtracted from the output score) -l val lval is a (positive) float (.01 precision) specifying penaltiy for lengthening a gap respectively (the penalties are subtracted from the output score) -a Causes the scoring matrix to be modified by dpal_set_ambiguity_codes. -e Causes the end postion of the alignment in both sequences to be printed. Do not confuse with the 'e' mode. -f1, -f2, -f3 Force specific implementations. -f2 forces use an implementation that might provide more informative error messages, possibly at the expense of some speed. -h Use a different scoring matrix: G and C matches = 3, A and T = 2, and mismatches = -0.5. (The default scoring matrix assigns 1 to a match, and -1 to a mismatch.) -p Causes the alignment to be displayed on stderr. -s causes only the score to printed. -m mval is the maximum allowed gap (default is 3). seq1 and seq2 are the sequences to be aligned. mode is one of g, G, l, or L specifying a global, global end-anchored, local, or local end-achored alignment respectively. For backward compatibility e is equivalent to G. REFERENCE
Please cite Rozen, S., Skaletsky, H. "Primer3 on the WWW for general users and for biologist programmers." In S. Krawetz and S. Misener, eds. Bioinformatics Methods and Protocols in the series Methods in Molecular Biology. Humana Press, Totowa, NJ, 2000, pages 365-386. SEE ALSO
primer3_core(1) oligotm(1) COPYRIGHT
Copyright (C) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://jura.wi.mit.edu/rozen), Helen Skaletsky All rights reserved. On Debian-based systems, please consult /usr/share/doc/primer3/copyright to read the licence of ntdpal. This manual page was written by Charles Plessy <plessy@debian.org> for the Debian(TM) system (but may be used by others). Permission is granted to copy, distribute and/or modify this document under the same terms as oligotm itself. ntdpal 1.1.4 05/09/2008 NTDPAL(1)

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KALIGN(1)							Kalign User Manual							 KALIGN(1)

NAME
kalign - performs multiple alignment of biological sequences. SYNOPSIS
kalign [infile.fasta] [outfile.fasta] [Options] kalign [-i infile.fasta] [-o outfile.fasta] [Options] kalign [< infile.fasta] [> outfile.fasta] [Options] DESCRIPTION
Kalign is a command line tool to perform multiple alignment of biological sequences. It employs the Muth?Manber string-matching algorithm, to improve both the accuracy and speed of the alignment. It uses global, progressive alignment approach, enriched by employing an approximate string-matching algorithm to calculate sequence distances and by incorporating local matches into the otherwise global alignment. OPTIONS
-s -gpo -gapopen -gap_open x Gap open penalty . -e -gpe -gap_ext -gapextension x Gap extension penalty. -t -tgpe -terminal_gap_extension_penalty x Terminal gap penalties. -m -bonus -matrix_bonus x A constant added to the substitution matrix. -c -sort <input, tree, gaps.> The order in which the sequences appear in the output alignment. -g -feature Selects feature mode and specifies which features are to be used: e.g. all, maxplp, STRUCT, PFAM-A? -same_feature_score Score for aligning same features. -diff_feature_score Penalty for aligning different features. -d -distance <wu, pair> Distance method -b -tree -guide-tree <nj, upgma> Guide tree method. -z -zcutoff Parameter used in the wu-manber based distance calculation. -i -in -input Name of the input file. -o -out -output Name of the output file. -a -gap_inc Increases gap penalties depending on the number of existing gaps. -f -format <fasta, msf, aln, clu, macsim> The output format. -q -quiet Print nothing to STDERR. Read nothing from STDIN. REFERENCES
o Timo Lassmann and Erik L.L. Sonnhammer (2005) Kalign - an accurate and fast multiple sequence alignment algorithm. BMC Bioinformatics 6:298 o Timo Lassmann, Oliver Frings and Erik L. L. Sonnhammer (2009) Kalign2: high-performance multiple alignment of protein and nucleotide sequences allowing external features. Nucleic Acid Research 3:858?865. AUTHORS
Timo Lassmann <timolassmann@gmail.com> Upstream author of Kalign. Charles Plessy <plessy@debian.org> Wrote the manpage. COPYRIGHT
Copyright (C) 2004, 2005, 2006, 2007, 2008 Timo Lassmann Kalign is free software. You can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation. This manual page was written by Charles Plessy <plessy@debian.org> for the Debian(TM) system (but may be used by others). Permission is granted to copy, distribute and/or modify this document under the same terms as kalign itself. On Debian systems, the complete text of the GNU General Public License version 2 can be found in /usr/share/common-licenses/GPL-2. kalign 2.04 February 25, 2009 KALIGN(1)
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