Hopefully someone here can point me in the correct direction.
I'm working on a username migration and am trying to map my users ols usernames to the new ones.
Right now every user has a username of firstname.lastname i.e. john.doe
I'm trying to create a bash or python script that will take... (3 Replies)
Hi,
I am having a file of dna sequences in fasta format which look like this:
>admin_1_45
atatagcaga
>admin_1_46
atatagcagaatatatat
with many such thousands of sequences in a single file. I want to the replace the accession Id "admin_1_45" similarly in following sequences to... (5 Replies)
I have two files. File1 is shown below.
>153L:B|PDBID|CHAIN|SEQUENCE
RTDCYGNVNRIDTTGASCKTAKPEGLSYCGVSASKKIAERDLQAMDRYKTIIKKVGEKLCVEPAVIAGIISRESHAGKVL
KNGWGDRGNGFGLMQVDKRSHKPQGTWNGEVHITQGTTILINFIKTIQKKFPSWTKDQQLKGGISAYNAGAGNVRSYARM
DIGTTHDDYANDVVARAQYYKQHGY
>16VP:A|PDBID|CHAIN|SEQUENCE... (7 Replies)
I have a fasta file as follows
>sp|O15090|FABP4_HUMAN Fatty acid-binding protein, adipocyte OS=Homo sapiens GN=FABP4 PE=1 SV=3
MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKN
TEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVM
KGVTSTRVYERA
>sp|L18484|AP2A2_RAT AP-2... (3 Replies)
Hi
How can I extract sequences from a fasta file with respect a certain criteria? The beginning of my file (containing in total more than 1000 sequences) looks like this:
>H8V34IS02I59VP
SDACNDLTIALLQIAREVRVCNPTFSFRWHPQVKDEVMRECFDCIRQGLG
YPSMRNDPILIANCMNWHGHPLEEARQWVHQACMSPCPSTKHGFQPFRMA... (6 Replies)
Hi,
I need some help with modifying fasta headers.
I have a fasta file with thousands of contigs and I need to modify their headers with the information obtained from a second file.
File 1 contains the fasta sequences:
>contig0001 length=11115 numreads=10777
agatgtagatctct... (6 Replies)
Hi,
I have a fasta file with multiple sequences. How can i get only unique sequences from the file.
For example
my_file.fasta
>seq1
TCTCAAAGAAAGCTGTGCTGCATACTGTACAAAACTTTGTCTGGAGAGATGGAGAATCTCATTGACTTTACAGGTGTGGACGGTCTTCAGAGATGGCTCAAGCTAACATTCCCTGACACACCTATAGGGAAAGAGCTAAC
>seq2... (3 Replies)
I could calculate the length of entire fasta sequences by following command,
awk '/^>/{if (l!="") print l; print; l=0; next}{l+=length($0)}END{print l}' unique.fasta
But, I need to calculate the length of a particular fasta sequence specified/listed in another txt file. The results to to be... (14 Replies)
I've been struggling with this one for quite a while and cannot seem to find a solution for this find/replace scenario. Perhaps I'm getting rusty.
I have a file that contains a number of metrics (exactly 3 fields per line) from a few appliances that are collected in parallel. To identify the... (3 Replies)
Hi,
I have to add 7 bases of specific nucleotide at the beginning and ending of all the fasta sequences of a file. For example, I have a multi fasta file namely test.fasta as given below
test.fasta
>TalAA18_Xoo_CIAT_NZ_CP033194.1:_2936369-2939570:+1... (1 Reply)
Discussion started by: dineshkumarsrk
1 Replies
LEARN ABOUT DEBIAN
devel::refcount
Devel::Refcount(3pm) User Contributed Perl Documentation Devel::Refcount(3pm)NAME
"Devel::Refcount" - obtain the REFCNT value of a referent
SYNOPSIS
use Devel::Refcount qw( refcount );
my $anon = [];
print "Anon ARRAY $anon has " . refcount($anon) . " reference
";
my $otherref = $anon;
print "Anon ARRAY $anon now has " . refcount($anon) . " references
";
DESCRIPTION
This module provides a single function which obtains the reference count of the object being pointed to by the passed reference value.
FUNCTIONS
$count = refcount($ref)
Returns the reference count of the object being pointed to by $ref.
COMPARISON WITH SvREFCNT
This function differs from "Devel::Peek::SvREFCNT" in that SvREFCNT() gives the reference count of the SV object itself that it is passed,
whereas refcount() gives the count of the object being pointed to. This allows it to give the count of any referent (i.e. ARRAY, HASH,
CODE, GLOB and Regexp types) as well.
Consider the following example program:
use Devel::Peek qw( SvREFCNT );
use Devel::Refcount qw( refcount );
sub printcount
{
my $name = shift;
printf "%30s has SvREFCNT=%d, refcount=%d
",
$name, SvREFCNT($_[0]), refcount($_[0]);
}
my $var = [];
printcount 'Initially, $var', $var;
my $othervar = $var;
printcount 'Before CODE ref, $var', $var;
printcount '$othervar', $othervar;
my $code = sub { undef $var };
printcount 'After CODE ref, $var', $var;
printcount '$othervar', $othervar;
This produces the output
Initially, $var has SvREFCNT=1, refcount=1
Before CODE ref, $var has SvREFCNT=1, refcount=2
$othervar has SvREFCNT=1, refcount=2
After CODE ref, $var has SvREFCNT=2, refcount=2
$othervar has SvREFCNT=1, refcount=2
Here, we see that SvREFCNT() counts the number of references to the SV object passed in as the scalar value - the $var or $othervar
respectively, whereas refcount() counts the number of reference values that point to the referent object - the anonymous ARRAY in this
case.
Before the CODE reference is constructed, both $var and $othervar have SvREFCNT() of 1, as they exist only in the current lexical pad. The
anonymous ARRAY has a refcount() of 2, because both $var and $othervar store a reference to it.
After the CODE reference is constructed, the $var variable now has an SvREFCNT() of 2, because it also appears in the lexical pad for the
new anonymous CODE block.
PURE-PERL FALLBACK
An XS implementation of this function is provided, and is used by default. If the XS library cannot be loaded, a fallback implementation in
pure perl using the "B" module is used instead. This will behave identically, but is much slower.
Rate pp xs
pp 225985/s -- -66%
xs 669570/s 196% --
SEE ALSO
o Test::Refcount - assert reference counts on objects
AUTHOR
Paul Evans <leonerd@leonerd.org.uk>
perl v5.14.2 2011-11-15 Devel::Refcount(3pm)