May I paraphrase your request: You want file2's sequences to prevail and file1's sequences inserted only if there's no equivalent in file2. If so, try
with any length fasta sequences. The traiing ">" can be taken care of piping through | sed '$d' if need be. Please be aware that your desired output's "Contig_98" line is missing an "AG" at the end.
Hi, I have two files where 1 contains data and the other contains strings eg
file 1
-0.00000 0.00000 0.00000
0.00000 0.00000 0.80000
0.50000 0.50000 0.60000
0.50000 0.50000 0.20000
-0.00000 0.00000 0.40000
file 2
F F F
F F F
T T T
T T T
T T T
How to I append file2 to file 1 to... (1 Reply)
I would like to extract the sequences larger than 10 bases but shorter than 18 along with the identifier from a FASTA file that looks like this:
> Seq I
ACGACTAGACGATAGACGATAGA
> Seq 2
ACGATGACGTAGCAGT
> Seq 3
ACGATACGAT
I know I can extract the IDs alone with the following code
grep... (3 Replies)
I have a fasta file that looks like this:
>Noname
ACCAAAATAATTCATGATATACTCAGATCCATCTGAGGGTTTCACCACTTGTAGAGCTAT
CAGAAGAATGTCAATCAACTGTCCGAGAAAAAAGAATCCCAGG
>Noname
ACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACTAGCACTAGCCTG
ACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGGACAAACAGA
...
I want to... (2 Replies)
I tried to write a script ( not working) to append first value from mylist to a file called my myfirstResult and to another called mysecondResult
awk ' {print $1} >> myfirsResult ' < mylist
awk ' {print $1} >> mysecondResult ' < mylist
$ cat mylist
A 02/16/2012
B 02/19/2012
C... (3 Replies)
Hey,
I've been trying to break a massive fasta formatted file into files containing each gene separately. Could anyone help me? I've tried to use the following code but i've recieved errors every time:
for i in *.rtf.out
do
awk '/^>/{f=++d".fasta"} {print > $i.out}' $i
done (1 Reply)
Hi All,
I have to append 2 lines at the end of a text file. If those 2 lines are already there then do not append else append the 2 lines to the text file.
Eg: I have a text file, file.txt
This text file might look like this,
/home/kp/make.jsp
/home/pk/model.jsp
I have to append... (1 Reply)
Hi frnds,
My requirement is I have a zip file with name say eg: test_ABC_UH_ccde2a_awdeaea_20150422.zip
within that there are subdirectories on each directory we again have .zip files and in that we have files like mama20150422.gz and so on.
Iam in need of a bash script so that it unzips... (0 Replies)
Hii,
Could someone help me to append string to the starting of all the filenames inside a directory but it should exclude .zip files and subdirectories.
Eg.
file1: test1.log
file2: test2.log
file3 test.zip
After running the script
file1: string_test1.log
file2: string_test2.log
file3:... (4 Replies)
2NDSCORE(1) User Contributed Documentation 2NDSCORE(1)NAME
2ndscore - find the best hairpin anchored at each position.
SYNOPSIS
2ndscore in.fasta > out.hairpins
DESCRIPTION
For every position in the sequence this will output a line:
-0.6 52 .. 62 TTCCTAAAGGTTCCA GCG CAAAA TGC CATAAGCACCACATT
(score) (start .. end) (left context) (hairpin) (right contenxt)
For positions near the ends of the sequences, the context may be padded with 'x' characters. If no hairpin can be found, the score will be
'None'.
Multiple fasta files can be given and multiple sequences can be in each fasta file. The output for each sequence will be separated by a
line starting with '>' and containing the FASTA description of the sequence.
Because the hairpin scores of the plus-strand and minus-strand may differ (due to GU binding in RNA), by default 2ndscore outputs two sets
of hairpins for every sequence: the FORWARD hairpins and the REVERSE hairpins. All the forward hairpins are output first, and are
identified by having the word 'FORWARD' at the end of the '>' line preceding them. Similarly, the REVERSE hairpins are listed after a '>'
line ending with 'REVERSE'. If you want to search only one or the other strand, you can use:
--no-fwd Don't print the FORWARD hairpins
--no-rvs Don't print the REVERSE hairpins
You can set the energy function used, just as with transterm with the --gc, --au, --gu, --mm, --gap options. The --min-loop, --max-loop,
and --max-len options are also supported.
FORMAT OF THE .BAG FILES
The columns for the .bag files are, in order:
1. gene_name
2. terminator_start
3. terminator_end
4. hairpin_score
5. tail_score
6. terminator_sequence
7. terminator_confidence: a combination of the hairpin and tail score that
takes into account how likely such scores are in a random sequence. This
is the main "score" for the terminator and is computed as described in
the paper.
8. APPROXIMATE_distance_from_end_of_gene: The *approximate* number of base
pairs between the end of the gene and the start of the terminator. This
is approximate in several ways: First, (and most important) TransTermHP
doesn't always use the real gene ends. Depending on the options you give
it may trim some off the ends of genes to handle terminators that
partially overlap with genes. Second, where the terminator "begins"
isn't that well defined. This field is intended only for a sanity check
(terminators reported to be the best near the ends of genes shouldn't be
_too far_ from the end of the gene).
USING TRANSTERM WITHOUT GENOME ANNOTATIONS
TransTermHP uses known gene information for only 3 things: (1) tagging the putative terminators as either "inside genes" or "intergenic,"
(2) choosing the background GC-content percentage to compute the scores, because genes often have different GC content than the intergenic
regions, and (3) producing slightly more readable output. Items (1) and (3) are not really necessary, and (2) has no effect if your genes
have about the same GC-content as your intergenic regions.
Unfortunately, TransTermHP doesn't yet have a simple option to run without an annotation file (either .ptt or .coords), and requires at
least 2 genes to be present. The solution is to create fake, small genes that flank each chromosome. To do this, make a fake.coords file
that contains only these two lines:
fakegene1 1 2 chome_id
fakegene2 L-1 L chrom_id
where L is the length of the input sequence and L-1 is 1 less than the length of the input sequence. "chrom_id" should be the word directly
following the ">" in the .fasta file containing your sequence. (If, for example, your .fasta file began with ">seq1", then chrom_id =
seq1).
This creates a "fake" annotation with two 1-base-long genes flanking the sequence in a tail-to-tail arrangement: --> <--. TransTermHP can
then be run with:
transterm -p expterm.dat sequence.fasta fake.coords
If the G/C content of your intergenic regions is about the same as your genes, then this won't have too much of an effect on the scores
terminators receive. On the other hand, this use of TransTermHP hasn't been tested much at all, so it's hard to vouch for its accuracy.
SEE ALSO transterm(1)AUTHOR
Alex Mestiashvili <alex@biotec.tu-dresden.de>
2011-02-19 2NDSCORE(1)