May I paraphrase your request: You want file2's sequences to prevail and file1's sequences inserted only if there's no equivalent in file2. If so, try
with any length fasta sequences. The traiing ">" can be taken care of piping through | sed '$d' if need be. Please be aware that your desired output's "Contig_98" line is missing an "AG" at the end.
Hi, I have two files where 1 contains data and the other contains strings eg
file 1
-0.00000 0.00000 0.00000
0.00000 0.00000 0.80000
0.50000 0.50000 0.60000
0.50000 0.50000 0.20000
-0.00000 0.00000 0.40000
file 2
F F F
F F F
T T T
T T T
T T T
How to I append file2 to file 1 to... (1 Reply)
I would like to extract the sequences larger than 10 bases but shorter than 18 along with the identifier from a FASTA file that looks like this:
> Seq I
ACGACTAGACGATAGACGATAGA
> Seq 2
ACGATGACGTAGCAGT
> Seq 3
ACGATACGAT
I know I can extract the IDs alone with the following code
grep... (3 Replies)
I have a fasta file that looks like this:
>Noname
ACCAAAATAATTCATGATATACTCAGATCCATCTGAGGGTTTCACCACTTGTAGAGCTAT
CAGAAGAATGTCAATCAACTGTCCGAGAAAAAAGAATCCCAGG
>Noname
ACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACTAGCACTAGCCTG
ACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGGACAAACAGA
...
I want to... (2 Replies)
I tried to write a script ( not working) to append first value from mylist to a file called my myfirstResult and to another called mysecondResult
awk ' {print $1} >> myfirsResult ' < mylist
awk ' {print $1} >> mysecondResult ' < mylist
$ cat mylist
A 02/16/2012
B 02/19/2012
C... (3 Replies)
Hey,
I've been trying to break a massive fasta formatted file into files containing each gene separately. Could anyone help me? I've tried to use the following code but i've recieved errors every time:
for i in *.rtf.out
do
awk '/^>/{f=++d".fasta"} {print > $i.out}' $i
done (1 Reply)
Hi All,
I have to append 2 lines at the end of a text file. If those 2 lines are already there then do not append else append the 2 lines to the text file.
Eg: I have a text file, file.txt
This text file might look like this,
/home/kp/make.jsp
/home/pk/model.jsp
I have to append... (1 Reply)
Hi frnds,
My requirement is I have a zip file with name say eg: test_ABC_UH_ccde2a_awdeaea_20150422.zip
within that there are subdirectories on each directory we again have .zip files and in that we have files like mama20150422.gz and so on.
Iam in need of a bash script so that it unzips... (0 Replies)
Hii,
Could someone help me to append string to the starting of all the filenames inside a directory but it should exclude .zip files and subdirectories.
Eg.
file1: test1.log
file2: test2.log
file3 test.zip
After running the script
file1: string_test1.log
file2: string_test2.log
file3:... (4 Replies)
BP_MASK_BY_SEARCH(1p) User Contributed Perl Documentation BP_MASK_BY_SEARCH(1p)NAME
mask_by_search - mask sequence(s) based on its alignment results
SYNOPSIS
mask_by_search.pl -f blast genomefile blastfile.bls > maskedgenome.fa
DESCRIPTION
Mask sequence based on significant alignments of another sequence. You need to provide the report file and the entire sequence data which
you want to mask. By default this will assume you have done a TBLASTN (or TFASTY) and try and mask the hit sequence assuming you've
provided the sequence file for the hit database. If you would like to do the reverse and mask the query sequence specify the -t/--type
query flag.
This is going to read in the whole sequence file into memory so for large genomes this may fall over. I'm using DB_File to prevent keeping
everything in memory, one solution is to split the genome into pieces (BEFORE you run the DB search though, you want to use the exact file
you BLASTed with as input to this program).
Below the double dash (--) options are of the form --format=fasta or --format fasta or you can just say -f fasta
By -f/--format I mean either are acceptable options. The =s or =n or =c specify these arguments expect a 'string'
Options:
-f/--format=s Search report format (fasta,blast,axt,hmmer,etc)
-sf/--sformat=s Sequence format (fasta,genbank,embl,swissprot)
--hardmask (booelean) Hard mask the sequence
with the maskchar [default is lowercase mask]
--maskchar=c Character to mask with [default is N], change
to 'X' for protein sequences
-e/--evalue=n Evalue cutoff for HSPs and Hits, only
mask sequence if alignment has specified evalue
or better
-o/--out/
--outfile=file Output file to save the masked sequence to.
-t/--type=s Alignment seq type you want to mask, the
'hit' or the 'query' sequence. [default is 'hit']
--minlen=n Minimum length of an HSP for it to be used
in masking [default 0]
-h/--help See this help information
AUTHOR - Jason Stajich
Jason Stajich, jason-at-bioperl-dot-org.
perl v5.14.2 2012-03-02 BP_MASK_BY_SEARCH(1p)