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Full Discussion: Sequence extraction
Top Forums Shell Programming and Scripting Sequence extraction Post 302951309 by Scrutinizer on Wednesday 5th of August 2015 03:39:48 AM
Old 08-05-2015
Maybe because FS is set to '\t' and there appear to be no TABs in your second sample file, so the fields will not match. And you are setting the index to the entire record, save the first character. which is probably not what you want (you probably meant to use $1 here, but that would not be a sure way to do it either, because in the FASTA format the identifier is allowed to contain spaces). And the FASTA file is word wrapped, so you need to take out the newlines and not use getline to get only the second line ....

The best way to do that is is to use ">" as a record separator and use "\n" as the field separator. By setting OFS as the empty string, and assigning a value to one of the fields, all newlines will be replaced by empty strings, so this will effectively remove the word wrap. And the sequence will become one continuous string, which will make it suitable for substring selection.

Using your file order, we would get something like this.
Code:
awk 'NR==FNR{i=$1; $1=x; A[i]=$0; next} $1 in A{print ">" $1 ORS substr(A[$1], $2, $3-$2+1)}' RS=\> FS='\n' OFS= file1 FS=" " RS="\n" file2


If we read the files the other way around, then it becomes more memory efficient:
Code:
awk 'NR==FNR{S[$1]=$2; E[$1]=$3; next} $1 in S{i=$1; $1=x; print RS i FS substr($0,S[i],E[i]-S[i]+1)}' file2 RS=\> FS='\n' OFS= file1


Last edited by Scrutinizer; 08-05-2015 at 04:52 AM..
 

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Ace::Sequence::Transcript(3pm)				User Contributed Perl Documentation			    Ace::Sequence::Transcript(3pm)

NAME
Ace::Sequence::Transcript - Simple "Gene" Object SYNOPSIS
# open database connection and get an Ace::Object sequence use Ace::Sequence; # get a megabase from the middle of chromosome I $seq = Ace::Sequence->new(-name => 'CHROMOSOME_I, -db => $db, -offset => 3_000_000, -length => 1_000_000); # get all the transcripts @genes = $seq->transcripts; # get the exons from the first one @exons = $genes[0]->exons; # get the introns @introns = $genes[0]->introns # get the CDSs (NOT IMPLEMENTED YET!) @cds = $genes[0]->cds; DESCRIPTION
Ace::Sequence::Gene is a subclass of Ace::Sequence::Feature. It inherits all the methods of Ace::Sequence::Feature, but adds the ability to retrieve the annotated introns and exons of the gene. OBJECT CREATION
You will not ordinarily create an Ace::Sequence::Gene object directly. Instead, objects will be created in response to a transcripts() call to an Ace::Sequence object. OBJECT METHODS
Most methods are inherited from Ace::Sequence::Feature. The following methods are also supported: exons() @exons = $gene->exons; Return a list of Ace::Sequence::Feature objects corresponding to annotated exons. introns() @introns = $gene->introns; Return a list of Ace::Sequence::Feature objects corresponding to annotated introns. cds() @cds = $gene->cds; Return a list of Ace::Sequence::Feature objects corresponding to coding sequence. THIS IS NOT YET IMPLEMENTED. relative() $relative = $gene->relative; $gene->relative(1); This turns on and off relative coordinates. By default, the exons and intron features will be returned in the coordinate system used by the gene. If relative() is set to a true value, then coordinates will be expressed as relative to the start of the gene. The first exon will (usually) be 1. SEE ALSO
Ace, Ace::Object, Ace::Sequence,Ace::Sequence::Homol, Ace::Sequence::Feature, Ace::Sequence::FeatureList, GFF AUTHOR
Lincoln Stein <lstein@cshl.org> with extensive help from Jean Thierry-Mieg <mieg@kaa.crbm.cnrs-mop.fr> Copyright (c) 1999, Lincoln D. Stein This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself. See DISCLAIMER.txt for disclaimers of warranty. POD ERRORS
Hey! The above document had some coding errors, which are explained below: Around line 168: You forgot a '=back' before '=head1' perl v5.14.2 2001-05-22 Ace::Sequence::Transcript(3pm)
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