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Full Discussion: Sequence extraction
Top Forums Shell Programming and Scripting Sequence extraction Post 302951309 by Scrutinizer on Wednesday 5th of August 2015 03:39:48 AM
Old 08-05-2015
Maybe because FS is set to '\t' and there appear to be no TABs in your second sample file, so the fields will not match. And you are setting the index to the entire record, save the first character. which is probably not what you want (you probably meant to use $1 here, but that would not be a sure way to do it either, because in the FASTA format the identifier is allowed to contain spaces). And the FASTA file is word wrapped, so you need to take out the newlines and not use getline to get only the second line ....

The best way to do that is is to use ">" as a record separator and use "\n" as the field separator. By setting OFS as the empty string, and assigning a value to one of the fields, all newlines will be replaced by empty strings, so this will effectively remove the word wrap. And the sequence will become one continuous string, which will make it suitable for substring selection.

Using your file order, we would get something like this.
Code:
awk 'NR==FNR{i=$1; $1=x; A[i]=$0; next} $1 in A{print ">" $1 ORS substr(A[$1], $2, $3-$2+1)}' RS=\> FS='\n' OFS= file1 FS=" " RS="\n" file2


If we read the files the other way around, then it becomes more memory efficient:
Code:
awk 'NR==FNR{S[$1]=$2; E[$1]=$3; next} $1 in S{i=$1; $1=x; print RS i FS substr($0,S[i],E[i]-S[i]+1)}' file2 RS=\> FS='\n' OFS= file1


Last edited by Scrutinizer; 08-05-2015 at 04:52 AM..
 

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Bio::ASN1::Sequence::Indexer(3pm)			User Contributed Perl Documentation			 Bio::ASN1::Sequence::Indexer(3pm)

NAME
Bio::ASN1::Sequence::Indexer - Indexes NCBI Sequence files. SYNOPSIS
use Bio::ASN1::Sequence::Indexer; # creating & using the index is just a few lines my $inx = Bio::ASN1::Sequence::Indexer->new( -filename => 'seq.idx', -write_flag => 'WRITE'); # needed for make_index call, but if opening # existing index file, don't set write flag! $inx->make_index('seq1.asn', 'seq2.asn'); my $seq = $inx->fetch('AF093062'); # Bio::Seq obj for Sequence (doesn't work yet) # alternatively, if one prefers just a data structure instead of objects $seq = $inx->fetch_hash('AF093062'); # a hash produced by Bio::ASN1::Sequence # that contains all data in the Sequence record PREREQUISITE
Bio::ASN1::Sequence, Bioperl and all dependencies therein. INSTALLATION
Same as Bio::ASN1::EntrezGene DESCRIPTION
Bio::ASN1::Sequence::Indexer is a Perl Indexer for NCBI Sequence genome databases. It processes an ASN.1-formatted Sequence record and stores the file position for each record in a way compliant with Bioperl standard (in fact its a subclass of Bioperl's index objects). Note that this module does not parse record, because it needs to run fast and grab only the gene ids. For parsing record, use Bio::ASN1::Sequence. As with Bio::ASN1::Sequence, this module is best thought of as beta version - it works, but is not fully tested. SEE ALSO
Please check out perldoc for Bio::ASN1::EntrezGene for more info. AUTHOR
Dr. Mingyi Liu <mingyi.liu@gpc-biotech.com> COPYRIGHT
The Bio::ASN1::EntrezGene module and its related modules and scripts are copyright (c) 2005 Mingyi Liu, GPC Biotech AG and Altana Research Institute. All rights reserved. I created these modules when working on a collaboration project between these two companies. Therefore a special thanks for the two companies to allow the release of the code into public domain. You may use and distribute them under the terms of the Perl itself or GPL (<http://www.gnu.org/copyleft/gpl.html>). CITATION
Liu, M and Grigoriev, A(2005) "Fast Parsers for Entrez Gene" Bioinformatics. In press OPERATION SYSTEMS SUPPORTED
Any OS that Perl & Bioperl run on. METHODS
fetch Parameters: $geneid - id for the Sequence record to be retrieved Example: my $hash = $indexer->fetch(10); # get Sequence #10 Function: fetch the data for the given Sequence id. Returns: A Bio::Seq object produced by Bio::SeqIO::sequence Notes: Bio::SeqIO::sequence does not exist and probably won't exist for a while! So call fetch_hash instead fetch_hash Parameters: $seqid - id for the Sequence record to be retrieved Example: my $hash = $indexer->fetch_hash('AF093062'); Function: fetch a hash produced by Bio::ASN1::Sequence for given id Returns: A data structure containing all data items from the Sequence record. Notes: Alternative to fetch() _file_handle Title : _file_handle Usage : $fh = $index->_file_handle( INT ) Function: Returns an open filehandle for the file index INT. On opening a new filehandle it caches it in the @{$index->_filehandle} array. If the requested filehandle is already open, it simply returns it from the array. Example : $fist_file_indexed = $index->_file_handle( 0 ); Returns : ref to a filehandle Args : INT Notes : This function is copied from Bio::Index::Abstract. Once that module changes file handle code like I do below to fit perl 5.005_03, this sub would be removed from this module perl v5.14.2 2005-05-04 Bio::ASN1::Sequence::Indexer(3pm)
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