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Top Forums Shell Programming and Scripting Filter (by max length) only lines not matching regex Post 302785161 by pathunkathunk on Monday 25th of March 2013 03:27:32 AM
Old 03-25-2013
Both of these work, thank you.

The problem with my original code was obscured by my choice of an example file with unrealistically short sequences. In reality my sequences are longer, and the problem with my code is that it captures headers along with short sequences.
 

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TFBS::PatternGen::SimplePFM(3pm)			User Contributed Perl Documentation			  TFBS::PatternGen::SimplePFM(3pm)

NAME
TFBS::PatternGen::SimplePFM - a simple position frequency matrix factory SYNOPSIS
my @sequences = qw( AAGCCT AGGCAT AAGCCT AAGCCT AGGCAT AGGCCT AGGCAT AGGTTT AGGCAT AGGCCT AGGCCT ); my $patterngen = TFBS::PatternGen::SimplePFM->new(-seq_list=>@sequences); my $pfm = $patterngen->pattern(); # $pfm is now a TFBS::Matrix::PFM object DESCRIPTION
TFBS::PatternGen::SimplePFM generates a position frequency matrix from a set of nucleotide sequences of equal length, The sequences can be passed either as strings, as Bio::Seq objects or as a fasta file. This pattern generator always creates only one pattern from a given set of sequences. new Title : new Usage : my $db = TFBS::PatternGen::SimplePFM->new(%args); Function: the constructor for the TFBS::PatternGen::SimplePFM object Returns : a TFBS::PatternGen::SimplePFM obkect Args : This method takes named arguments; you must specify one of the following -seq_list # a reference to an array of strings # and/or Bio::Seq objects # or -seq_stream # A Bio::SeqIO object # or -seq_file # the name of the fasta file containing # all the sequences pattern all_patterns patternSet The three above methods are used fro the retrieval of patterns, and are common to all TFBS::PatternGen::* classes. Please see TFBS::PatternGen for details. perl v5.14.2 2008-01-24 TFBS::PatternGen::SimplePFM(3pm)
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