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Top Forums Shell Programming and Scripting Filter (by max length) only lines not matching regex Post 302785161 by pathunkathunk on Monday 25th of March 2013 03:27:32 AM
Old 03-25-2013
Both of these work, thank you.

The problem with my original code was obscured by my choice of an example file with unrealistically short sequences. In reality my sequences are longer, and the problem with my code is that it captures headers along with short sequences.
 

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Bio::Tools::Analysis::Protein::ELM(3pm) 		User Contributed Perl Documentation		   Bio::Tools::Analysis::Protein::ELM(3pm)

NAME
Bio::Tools::Analysis::Protein::ELM - a wrapper around the ELM server which predicts short functional motifs on amino acid sequences SYNOPSIS
# get a Bio::Seq object to start with, or a Bio::PrimaryI object. my $tool = Bio::Tools::Analysis::Protein::ELM-> new(seq => $seqobj->primary_seq() ); $tool->compartment(['ER', 'Golgi']); $tool->species(9606); $tool->run; my @fts = $tool->Result('Bio::SeqFeatureI'); $seqobj->addSeqFeature(@fts); DESCRIPTION
This module is a wrapper around the ELM server <http://elm.eu.org/> which predicts short functional motifs on amino acid sequences. False positives can be limited by providing values for the species and cellular compartment of the protein. To set the species attribute, use either a Bio::Species object or an NCBI taxon ID number. To set the cell compartment attribute (any number of compartments can be chosen) use an array reference to a list of compartment names. Results can be obtained either as raw text output, parsed into a data structure, or as Bio::SeqFeature::Generic objects. SEE ALSO
Bio::SimpleAnalysisI, Bio::WebAgent FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHORS
Richard Adams, Richard.Adams@ed.ac.uk, APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ compartment name : compartment usage : $elm->compartment(['golgi', 'er']); purpose : get/setter for cell compartment specifications arguments : None, single compartment string or ref to array of compartment names. returns : Array of compartment names (default if not previously set). species name : species usage : $tool->species('9606'); purpose : get/setter for species selction for ELM server arguments : none, taxon_id or Bio::Species object returns : a string of the ncbi taxon_id result name : result usage : $tool->result('Bio::SeqFeatureI'); purpose : parse results into sequence features or basic data format arguments : 1. none (retrieves raw text without html) 2. a value (retrieves data structure) 3. 'Bio::SeqFeatureI' (returns array of sequence features) tag names are : {method => 'ELM', motif => motifname, peptide => seqeunce of match, concensus => regexp of match}. returns : see arguments. perl v5.14.2 2012-03-02 Bio::Tools::Analysis::Protein::ELM(3pm)
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