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Top Forums Shell Programming and Scripting grep pattern in a file not working. please help... Post 302394783 by Zoho on Friday 12th of February 2010 01:38:00 PM
Old 02-12-2010
Fantastic! Turns out it is the format difference. This seems a typical problem between unix and msdos txt file. Thanks, guys!
 

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TQS(1)							      General Commands Manual							    TQS(1)

NAME
tqs - Trim Quality Solexa-Illumina Sequences SYNOPSIS
Quality trim solexa-Illumina sequence reads using user-defined thresholds. OPTIONS
-h, --help show this help message and exit -f SEQFILE, --sequence file=SEQFILE Illumina sequence file - Output format from the 1G Genome Analyzer (_seq.txt): 7 1 255 669 AACCCCCACTCCTACAACGCCATCATTCCCCTCGAC -q QUALFILE, --qual file=QUALFILE A prb file containing all the Illumina intensities, as outputted by the 1G Genome Analyzer (_prb.txt) -l MER, --length=MER Length of sequence reads (i.e. Number of sequencing cycles, default=36) -t THRESHOLD, --threshold=THRESHOLD Base intensity threshold value (-40 to 40, default=5) -d DIFF, --difference=DIFF Base intensity difference between top intensity and second best (1 to 80, default=5) -c CONSEC, --consec=CONSEC Minimum number of consecutive bases passing threshold values (default=20) -v, --verbose Runs in Verbose mode. SEE ALSO
/usr/share/doc/ssake/TQS.readme AUTHORS
This manual page was written by Andreas Tille <tille@debian.org> for the Debian system (but may be used by others). Permission is granted to copy, distribute and/or modify this document under the terms of the GNU General Public License, Version 2 any later version published by the Free Software Foundation. On Debian systems, the complete text of the GNU General Public License can be found in /usr/share/common-licenses/GPL. January 2008 TQS(1)
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