Tough Substituion command Post: 302233489

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Top Forums UNIX for Advanced & Expert Users Tough Substituion command Post 302233489 by ggggdddd on Sunday 7th of September 2008 10:46:30 PM

09-07-2008

Registered User

Quote:

Originally Posted by BMDan

Not clear why there's a colon at the start; is this vim? Here's how to do it in vim:

Code:

:%s/Mg\([24]\).\(q[np]s[b]?\) W=0\.175u/Mg\1.\2 W=wmg\1\2/g

Thanks!
I am using Vi.
I tried using the command but it did not execute. (Even tried in vim)
I tried searching for
/Mg$[24]$.$q[np][as]$

and it could find all the lines.

But when I search for

/Mg$[24]$.$q[np][as][b]?$ It cannot find anything. How can I make the presence of that last charachter b optional in the substitution command?

One more question: How do I substitute in case I have some charachters in between Mg...... and W=175u like below. These in between charachters should not be disturbed.

Mg2.qns A B C L=123u W=0.175u
Mg2.qpsb DE 12 c1 L=123u W=0.175u
Mg4.qns M N O W=0.175u
Mg4.qpsb W=0.175u

Last edited by ggggdddd; 09-08-2008 at 12:06 AM.. Reason: More details added

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perlprimer

PERLPRIMER(1)						      General Commands Manual						     PERLPRIMER(1)

NAME

       perlprimer - graphically specify amplicon of DNA or mRNA sequences and design primers

SYNOPSIS

       perlprimer

DESCRIPTION

       PerlPrimer  calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. To adjust for
       the salt conditions of the PCR, PerlPrimer uses the empirical formula derived by von Ahsen, et al. (2001) and allows the  user  to  specify
       the  concentration of Mg2+, dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting
       temperature, giving rise to a maximum yeild of product when amplified.	PerlPrimer is written in Perl and requires Perl/Tk.  In  addition,
       for  QPCR  functionality PerlPrimer requires the open-source Spidey executable from NCBI. The program is designed to be cross-platform com-
       patible and has been developed and tested on both Microsoft Windows and GNU/Linux-based operating systems. Users have also reported success
       using the program under Mac OS X.

SEE ALSO

	A very nice tutorial on http://perlprimer.sourceforge.net is also distributed as a debian package perlprimer-doc.

AUTHOR

       perlprimer was written by Owen Marshall <owenjm@users.sourceforge.net>

       This manual page was assembled by Steffen Moeller <steffen_moeller@gmx.de>, for the Debian project (but may be used by others).

								  6 January 2005						     PERLPRIMER(1)