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Special Forums News, Links, Events and Announcements Software Releases - RSS News Basic Local Alignment Search Tool 2.2.19 (Default branch)
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Old 12-18-2008
Basic Local Alignment Search Tool 2.2.19 (Default branch)
BLAST is a set of similarity search programs designed to explore all of the available sequence databases regardless of whether the query is protein or DNA. It uses a heuristic algorithm which seeks local as opposed to global alignments, and is therefore able to detect relationships among sequences which share only isolated regions of similarity. It can be run locally as a full executable, and can be used to run BLAST searches against private, local databases, or downloaded copies of the NCBI databases. It runs on Mac OS, Win32, LINUX, Solaris, IBM AIX, SGI, Compaq OSF, and HP- UX systems. License: Open Software License Changes:
The BLASTDB environment variable now supports multiple database search paths. When possible, a smaller protein lookup table is used to improve performance. formatrpsdb now supports creating databases larger than 2G. seedtop now supports searches with gi lists. The X3 value for blastn/megablast was corrected. Image

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LEARN ABOUT DEBIAN

bp_mask_by_search

BP_MASK_BY_SEARCH(1p)					User Contributed Perl Documentation				     BP_MASK_BY_SEARCH(1p)

NAME

       mask_by_search - mask sequence(s) based on its alignment results

SYNOPSIS

	 mask_by_search.pl -f blast genomefile blastfile.bls > maskedgenome.fa

DESCRIPTION

       Mask sequence based on significant alignments of another sequence.  You need to provide the report file and the entire sequence data which
       you want to mask.  By default this will assume you have done a TBLASTN (or TFASTY) and try and mask the hit sequence assuming you've
       provided the sequence file for the hit database.  If you would like to do the reverse and mask the query sequence specify the -t/--type
       query flag.

       This is going to read in the whole sequence file into memory so for large genomes this may fall over.  I'm using DB_File to prevent keeping
       everything in memory, one solution is to split the genome into pieces (BEFORE you run the DB search though, you want to use the exact file
       you BLASTed with as input to this program).

       Below the double dash (--) options are of the form --format=fasta or --format fasta or you can just say -f fasta

       By -f/--format I mean either are acceptable options.  The =s or =n or =c specify these arguments expect a 'string'

       Options:
	   -f/--format=s    Search report format (fasta,blast,axt,hmmer,etc)
	   -sf/--sformat=s  Sequence format (fasta,genbank,embl,swissprot)
	   --hardmask	    (booelean) Hard mask the sequence
			    with the maskchar [default is lowercase mask]
	   --maskchar=c     Character to mask with [default is N], change
			    to 'X' for protein sequences
	   -e/--evalue=n    Evalue cutoff for HSPs and Hits, only
			    mask sequence if alignment has specified evalue
			    or better
	   -o/--out/
	   --outfile=file   Output file to save the masked sequence to.
	   -t/--type=s	    Alignment seq type you want to mask, the
			    'hit' or the 'query' sequence. [default is 'hit']
	   --minlen=n	    Minimum length of an HSP for it to be used
			    in masking [default 0]
	   -h/--help	    See this help information

AUTHOR - Jason Stajich
       Jason Stajich, jason-at-bioperl-dot-org.

perl v5.14.2							    2012-03-02						     BP_MASK_BY_SEARCH(1p)