Eliminating sequences based on Distances


 
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# 1  
Old 05-26-2013
Eliminating sequences based on Distances

I have to remove sequences from a file based on the distance value. I am attaching the file containing the distances (Distance.xls)

The second file looks something like this:

Sequences.txt
Code:
 
>Sample1 Freq 59
ggatatgatgatgaactggt
>Sample1 Freq 54
ggatatgatgttgaactggt
>Sample1 Freq 44
ggatatgttgatgaactggt
>Sample1 Freq 39
ggatttgatgatgaactggt
>Sample1 Freq 3a 39
ggatatgatgatgaactggt
>Sample2 Freq 38
ggatatgatgatgaactggt
>Sample2 run13 reversed 7 3a Freq 32
ggatttgatgatgaactggt
>Sample2 run13 reversed 8 3a 30
ggatttgatgatgaactggt
>Sample2 29
ggatatgatgatgaactcct
>Sample2 reversed 10 3a 27
ggatatgatgatgaactggt

If the distance value is below 1, I need to remove the sequences from file Sequences.txt listed on colum Species 2. Thus, the output file should look something like this:

Outputfile
Code:
 
>Sample1 Freq 59
ggatatgatgatgaactggt
>Sample1 Freq 54
ggatatgatgttgaactggt
>Sample1 Freq 44
ggatatgttgatgaactggt
>Sample1 Freq 39
ggatttgatgatgaactggt
>Sample1 Freq 3a 39
ggatatgatgatgaactggt
>Sample2 29
ggatatgatgatgaactcct

I am trying to get an AWK or SED script so I can better understand the syntax. I am not that familiar with Perl but I might be able to follow the syntax once I see the script.
Thanks!
# 2  
Old 05-26-2013
None of the core UNIX utilities can read an xls file. It is purely a a windows-ism. A comma-delimited file, sure UNIX can read it. You need to convert your example to a csv (or tab-delimited), or just post a copy of the csv, or tab-delimited file.
# 3  
Old 05-26-2013
TXT file uploaded
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