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If what you're saying is that you want to make both of the above changes to that file, please make both changes in one invocation of awk instead of invoking awk twice. You will get your results...

I think juggernautjoee is looking for something more like:
awk '/^02,193631306,000300032,1,/{$0 = substr($0,1,32) "2400" substr($0,37)}1' file
or if the line to be changed is always line #2:
awk...