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fahash(1) [debian man page]

FAHASH(1)							   User Commands							 FAHASH(1)

NAME
fahash - sequence hash builder for reverse e-PCR SYNOPSIS
fahash [-hV] -b hash-file [-w wdsize] [-f period] [-F fragment_min,fragment_max] [-k] [-c cachesize] [-v 1|2] famap-file ... fahash [-hV] -T hash-file [-o outfile] DESCRIPTION
The program fahash is part of the e-PCR suite and is used to build hash-files, and output word usage statistics for reverse e-PCR searches. OPTIONS
-b hash-file Build hash tables (hash-file) from sequence files, -T hash-file Print word usage statistics for hash-file -o outfile Write output to file `outfile' build-options: -w wordsize Set word size when building hash tables -f period Set discontiguity when building hash tables -k Skip repeats when building hash-file -F min,max Set watermarks for fragment size (in Mb) (version 1 only) -c cachesize Set cache size (version 2 only) -v ver Use format version (1|2, 2 is default) EXAMPLE
famap -tN -b genome.famap org/chr_*.fa fahash -b genome.hash -w 12 -f3 ${PWD}/genome.famap re-PCR -s genome.hash -n1 -g1 ACTATTGATGATGA AGGTAGATGTTTTT 120-200 See famap(1) and re-pcr(1) SEE ALSO
/usr/share/doc/ncbi-epcr/README.txt bioperl(1), e-pcr(1), famap(1) and re-pcr(1) AUTHORS
This manual page was written by Andreas Tille <tille@debian.org> for the Debian system (but may be used by others). Permission is granted to copy, distribute and/or modify this document under the terms of the GNU General Public License, Version 2 any later version published by the Free Software Foundation. On Debian systems, the complete text of the GNU General Public License can be found in /usr/share/common-licenses/GPL. Reverse e-PCR: sequence hash builder version 2.3.9 April 2008 FAHASH(1)

Check Out this Related Man Page

RE-PCR(1)						      General Commands Manual							 RE-PCR(1)

NAME
re-PCR -- Find sequence tagged sites (STS) in DNA sequences SYNOPSIS
re-PCR [-hV] -p hash-file [-g gaps] [-n mism] [-lq] [primer ...] re-PCR [-hV] -P hash-file [-g gaps] [-n mism] [-l] [-m margin] [-O+|-] [-C batchcnt] [-o outfile] [-r+|-] [primers-file ...] re-PCR [-hV] -s hash-file [-g gaps] [-n mism] [-lq] [-m margin] [-o outfile] [-r+|-] [left right lo[-hi] [...]] re-PCR [-hV] -S hash-file [-g gaps] [-n mism] [-lq] [-m margin] [-O+|-] [-C batchcnt] [-o outfile] [-r+|-] [stsfile ...] DESCRIPTION
Implements reverse searching (called Reverse e-PCR) to make it feasible to search the human genome sequence and other large genomes by per- forming STS and primer searches. OPTIONS
-p=hash-file Perform primer lookup using hash-file -P=hash-file Perform primer lookup using hash-file -s=hash-file Perform STS lookup using hash-file -S=hash-file Perform STS lookup using hash-file -n=mism Set max allowed mismatches per primer for lookup -g=gaps Set max allowed indels per primer for lookup -m=margin Set variability for STS size for lookup -l Use presize alignments (only if gaps>0) -G Print alignments in comments -d=min-max Set default STS size -r=+|- Enable/disable reverse STS lookup -O=+|- Enable/disable syscall optimisation -C=batchcnt Set number of STSes per batch -o=outfile Set output file name -q Quiet (no progress indicator) EXAMPLE
famap -tN -b genome.famap org/chr_*.fa fahash -b genome.hash -w 12 -f3 ${PWD}/genome.famap re-PCR -s genome.hash -n1 -g1 ACTATTGATGATGA AGGTAGATGTTTTT 120-200 See famap(1) and fahash(1) SEE ALSO
/usr/share/doc/ncbi-epcr/README.txt bioperl(1), e-pcr(1), famap(1) and fahash(1) AUTHORS
This manual page was written by Andreas Tille <tille@debian.org> for the Debian system (but may be used by others). Permission is granted to copy, distribute and/or modify this document under the terms of the GNU General Public License, Version 2 any later version published by the Free Software Foundation. On Debian systems, the complete text of the GNU General Public License can be found in /usr/share/common-licenses/GPL. April 2008 RE-PCR(1)

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