10-28-2002
A quick suggestion - post the script - makes trouble shooting easier (for myself anyways).
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LEARN ABOUT DEBIAN
theseus_align
THESEUS_ALIGN(1) General Commands Manual THESEUS_ALIGN(1)
NAME
theseus_align - quick-and-dirty way to superimpose proteins
SYNOPSIS
theseus_align [theseus options] -f pdbfile1.pdb pdbfile2.pdb ...
OPTIONS
The options given to the script will be passed on to theseus. For a complete description, see the man page for theseus (1).
DESCRIPTION
This manual page briefly documents briefly the script theseus_align, designed for a quick-and-dirty way to ML superposition proteins with
different sequences. It should work very well when the protein sequences are relatively similar, although the ML method will still give
much better results than least-squares when the sequences are moderately divergent. Technically, this procedure gives a structure-based
superposition of a sequence-based alignment. It does not perform a structure-based alignment.
First, the script uses theseus to create FASTA formatted sequence files corresponding to the exact protein sequences found in the pdb files
that you supply.
Second, these sequences are aligned using the multiple sequence alignment program of your choice. The script can easily be modified for
CLUSTALW, T_COFFEE, KALIGN, DIALIGN2, or MAFFT. Any multiple sequence alignment program can be used, as long as it can generate clustal-
formatted files. However, I highly recommend Bob Edgar's MUSCLE program for both its speed and accuracy. (For more info see
http://www.drive5.com/muscle/ .)
Third, theseus performs a superposition of the structures using the sequence alignment as a guide.
The installed version of theseus_align uses muscle (1) for doing the multiple sequence alignment. If you wish to use one of the other pro-
grams mentioned above, you'll have to copy the script to your own directory and edit it.
SEE ALSO
theseus (1), muscle (1), clustalw (1), t_coffee (1), kalign (1), dialign2 (1), mafft (1). All of these programs can be installed on Debian
or Ubuntu systems using apt-get (8).
AUTHOR
theseus_align was written by Douglas L. Theobald, Department of Biochemistry, Brandeis University.
November, 2008 THESEUS_ALIGN(1)