How to add specific bases at the beginning and ending of all the fasta sequences?
Hi,
I have to add 7 bases of specific nucleotide at the beginning and ending of all the fasta sequences of a file. For example, I have a multi fasta file namely test.fasta as given below
I need to add the following bases CTGATAGTA at the beginning and ending of every fasta sequences respectively.
I have tried the following command line to add bases at the end of fasta file, but it is not serving my purpose,
. For adding bases at the beginning I do not have any idea, However, I tried the following command
, but its adding bases at the fasta header.
Please help me to do the same.
Thank you.
I have got an Perl array like:
@array = (1,2,3,4,5,6,1,2,3,4,1,2,1,2,3,4,5,6,7,8,9...............)
This numeric sequence will be always sequentially increasing, unless it encounters, The beginning of the new sequentially increasing numeric sequence.
SO in this array we get sequentially... (5 Replies)
Hi, I have multiple large files which consist of the below format:
I am trying to write an awk or sed script to remove all occurrences of the 00 record except the first and remove all of the 80 records except the last one.
Any help would be greatly appreciated. (10 Replies)
Hi,
I am having a file of dna sequences in fasta format which look like this:
>admin_1_45
atatagcaga
>admin_1_46
atatagcagaatatatat
with many such thousands of sequences in a single file. I want to the replace the accession Id "admin_1_45" similarly in following sequences to... (5 Replies)
I have two files. File1 is shown below.
>153L:B|PDBID|CHAIN|SEQUENCE
RTDCYGNVNRIDTTGASCKTAKPEGLSYCGVSASKKIAERDLQAMDRYKTIIKKVGEKLCVEPAVIAGIISRESHAGKVL
KNGWGDRGNGFGLMQVDKRSHKPQGTWNGEVHITQGTTILINFIKTIQKKFPSWTKDQQLKGGISAYNAGAGNVRSYARM
DIGTTHDDYANDVVARAQYYKQHGY
>16VP:A|PDBID|CHAIN|SEQUENCE... (7 Replies)
I have a fasta file as follows
>sp|O15090|FABP4_HUMAN Fatty acid-binding protein, adipocyte OS=Homo sapiens GN=FABP4 PE=1 SV=3
MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKN
TEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVM
KGVTSTRVYERA
>sp|L18484|AP2A2_RAT AP-2... (3 Replies)
Hi
How can I extract sequences from a fasta file with respect a certain criteria? The beginning of my file (containing in total more than 1000 sequences) looks like this:
>H8V34IS02I59VP
SDACNDLTIALLQIAREVRVCNPTFSFRWHPQVKDEVMRECFDCIRQGLG
YPSMRNDPILIANCMNWHGHPLEEARQWVHQACMSPCPSTKHGFQPFRMA... (6 Replies)
Hi,
I have a fasta file with multiple sequences. How can i get only unique sequences from the file.
For example
my_file.fasta
>seq1
TCTCAAAGAAAGCTGTGCTGCATACTGTACAAAACTTTGTCTGGAGAGATGGAGAATCTCATTGACTTTACAGGTGTGGACGGTCTTCAGAGATGGCTCAAGCTAACATTCCCTGACACACCTATAGGGAAAGAGCTAAC
>seq2... (3 Replies)
I could calculate the length of entire fasta sequences by following command,
awk '/^>/{if (l!="") print l; print; l=0; next}{l+=length($0)}END{print l}' unique.fasta
But, I need to calculate the length of a particular fasta sequence specified/listed in another txt file. The results to to be... (14 Replies)
I have a fasta file as follows
>sp|Q8WWQ8|STAB2_HUMAN Stabilin-2 OS=Homo sapiens OX=9606 GN=STAB2 PE=1 SV=3
MMLQHLVIFCLGLVVQNFCSPAETTGQARRCDRKSLLTIRTECRSCALNLGVKCPDGYTM
ITSGSVGVRDCRYTFEVRTYSLSLPGCRHICRKDYLQPRCCPGRWGPDCIECPGGAGSPC
NGRGSCAEGMEGNGTCSCQEGFGGTACETCADDNLFGPSCSSVCNCVHGVCNSGLDGDGT... (3 Replies)
I have to mine the following sequence pattern from a large fasta file namely gene.fasta (contains multiple fasta sequences) along with the flanking sequences of 5 bases at starting position and ending position,
AAGCZ-N16-AAGCZ
Z represents A, C or G (Except T)
N16 represents any of the four... (3 Replies)
Discussion started by: dineshkumarsrk
3 Replies
LEARN ABOUT DEBIAN
sigma
SIGMA(1) Manual of Sigma SIGMA(1)NAME
sigma - Simple greedy multiple alignment of non-coding DNA sequences
SYNOPSIS
sigma [options] [inputfile.fasta] [inputfile2.fasta ...]
Each fasta file may contain a single sequence or multiple sequences; all sequences will be aligned together.
DESCRIPTION
Sigma ("Simple greedy multiple alignment") is an alignment program with a new algorithm and scoring scheme designed specifically for
non-coding DNA sequence. It uses a strategy of seeking the best possible gapless local alignments, at each step making the best possible
alignment consistent with existing alignments, and scores the significance of the alignment based on the lengths of the aligned fragments
and a background model which may be supplied or estimated from an auxiliary file of intergenic DNA. With real data, while "correctness"
can't be directly quantified for the alignment, running the PhyloGibbs motif finder on pre-aligned sequence suggests that Sigma's
alignments are superior.
OPTIONS -A --aligned_output
Aligned, pretty-printed output (compare with -F option) (default: only this). See also -C.
-b --bgprobfile filename
Auxiliary file (in fasta format) from which to read background sequences (overridden by -B). Typically this is a file containing large
quantities of similar non-coding sequence, from which background probabilities of single- and di-nucleotides may be estimated.
-B --bgseqfile filename
File containing background probabilities. The format is described further below.
-C --caps_only
Use only upper-case letters in output sequence, for compatibility with output of some other programs like ClustalW and MLagan. By
default, output is mixed-case (as in Dialign), and lower-case bases are treated as not aligned.
-F --fasta_output
Multi-fasta output (can use both -A and -F in either order). See also -C.
-n --ncorrel number
Background correlation (default 2=dinucleotide; 1=single-site basecounts, 0=0.25 per base).
-x, --significance number
Set limit for how probable the match is by chance (default 0.002, smaller=more stringent).
-h, --help
Displays this list of options.
MORE HELP
The "significance" parameter (-x) determines whether local alignments are accepted or rejected. The default at present is 0.002.
Experiments on synthetic data (described in the paper) suggest that 0.002 is about the threshold where sigma fails to align
phylogenetically-unrelated data that has moderate (yeast-like) dinucleotide correlation.
Using a "background model" appropriate to the sequences being aligned greatly reduces spurious alignments on synthetic data (and, one
hopes, on real data too). The simplest way to ensure this is to supply, via the -b parameter, a FASTA-format file containing large
quantities of similar sequence data (eg, if one is aligning yeast sequences, supply a file containing all intergenic yeast sequence).
Instead of this, if the single-site and dinucleotide frequencies are known already, they may be supplied in a file via the -B option. The
file format should be: one entry per line, with the mononucleotide or dinucleotide (case-insensitive) followed by the frequency. (eg, "A
0.3", "AT 0.16", etc on successive lines.) A sample file is in the "Background" subdirectory of the source distribution (on Debian systems,
this file can be found in the /usr/share/doc/sigma-align/Background directory). A file like "yeast.nc.3.freq" in the "tests" subdirectory
of the MEME source distribution works fine (trinucleotide counts are ignored).
REFERENCE
Please cite Sigma: Rahul Siddharthan (2006) Multiple alignment of weakly-conserved non-coding DNA sequence BMC Bioinformatics 2006, 7:143
doi:10.1186/1471-2105-7-143 Published 16 March 2006, available online at http://www.biomedcentral.com/1471-2105/7/143/
AUTHORS
Rahul Siddharthan <rsidd@imsc.res.in>
Wrote sigma. If you're using Sigma for actual research, please let the author know so that he can alert you of bugfixes or new releases.
Charles Plessy <charles-debian-nospam@plessy.org>
Wrote the manpage in DocBook XML for the Debian distribution.
COPYRIGHT
Copyright (C) 2006-2007 Rahul Siddharthan
Copyright (C) 2006-2007 Charles Plessy
Sigma is free software. You can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free
Software Foundation.
On Debian systems, the complete text of the GNU General Public License can be found in /usr/share/common-licenses/GPL.
sigma 1.1 2007-04-07 SIGMA(1)