Dear Rudic,
Below script can rename the characters in gene1.fasta specified in list.txt.
However, it is not based on the position. Its purely based on the matching strings between the two files. But, here my problem is different, I tried workout like this
, but my list.txt is not having common strings, so I can not rename sequentially. That is why I seek your help.
Last edited by dineshkumarsrk; 11-13-2019 at 04:12 AM..
Hi ,
I have a typical situation. I have 4 files and with different headers (number of headers is varible ).
I need to make such a merged file which will have headers combined from all files (comman coluns should appear once only).
For example -
File 1
H1|H2|H3|H4
11|12|13|14
21|22|23|23... (1 Reply)
Hi Guys,
While I was writing one shell script , I just got struck at this point.
I need to extract words from a file at some specified position and do some comparison operation and need to replace the extracted word with another word.
Eg : I like Orange very much.
I need to replace... (19 Replies)
Hi,
I am new to unix. I want to delete 2 words placed at position say for example at 23rd and 45th position in a line. I used sed but couldnt achieve this.
Example: the file contains 2 lines
12345 98765 "12345" 876
12345 98765 "64578" 876
I want to delete " placed at position 13 and 19... (4 Replies)
I have a file with thousands of sequences that looks like this:
I need to replace the headers using a second file
Thus, I will end up having the following file:
I am looking for an AWK script that I can easily plug in my current pipeline.
Any help will be greatly appreciated! (6 Replies)
Hi, I have a file1 of many long sequences, each preceded by a unique header line. file2 is 3-columns list: headers name, start position, end position. I'd like to extract the sequence region of file1 specified in file2.
Based on a post elsewhere, I found the code:
awk... (2 Replies)
Hi,
I am unable to find the right option to extract the data in the fixed width file.
sample data
abcd1234xgyhsyshijfkfk
hujk9876 io xgla
loki8787eljuwoejroiweo
dkfj9098 dja
Search based on position 8-9="xg" and print the entire row
output
... (4 Replies)
OS : Linux 2.6x
Shell : Korn
In a single file , how can I identify all the Uniqe values at a specific character position and length of each record ,
and simultaneously SPLIT the records of the file based on each of these values and write them in seperate files .
Lets say :
a) I want to... (4 Replies)
I have two files. File1 is shown below.
>153L:B|PDBID|CHAIN|SEQUENCE
RTDCYGNVNRIDTTGASCKTAKPEGLSYCGVSASKKIAERDLQAMDRYKTIIKKVGEKLCVEPAVIAGIISRESHAGKVL
KNGWGDRGNGFGLMQVDKRSHKPQGTWNGEVHITQGTTILINFIKTIQKKFPSWTKDQQLKGGISAYNAGAGNVRSYARM
DIGTTHDDYANDVVARAQYYKQHGY
>16VP:A|PDBID|CHAIN|SEQUENCE... (7 Replies)
Hi,
I have a file with multiple lines(fixed width dat file). I want to search for '02' in the positions 45-46 and if available, in that lines, I need to replace value in position 359 with blank. As I am new to unix, I am not able to figure out how to do this. Can you please help me to achieve... (9 Replies)
Discussion started by: Pradhikshan
9 Replies
LEARN ABOUT DEBIAN
kalign
KALIGN(1) Kalign User Manual KALIGN(1)NAME
kalign - performs multiple alignment of biological sequences.
SYNOPSIS
kalign [infile.fasta] [outfile.fasta] [Options]
kalign [-i infile.fasta] [-o outfile.fasta] [Options]
kalign [< infile.fasta] [> outfile.fasta] [Options]
DESCRIPTION
Kalign is a command line tool to perform multiple alignment of biological sequences. It employs the Muth?Manber string-matching algorithm,
to improve both the accuracy and speed of the alignment. It uses global, progressive alignment approach, enriched by employing an
approximate string-matching algorithm to calculate sequence distances and by incorporating local matches into the otherwise global
alignment.
OPTIONS -s -gpo -gapopen -gap_open x
Gap open penalty .
-e -gpe -gap_ext -gapextension x
Gap extension penalty.
-t -tgpe -terminal_gap_extension_penalty x
Terminal gap penalties.
-m -bonus -matrix_bonus x
A constant added to the substitution matrix.
-c -sort <input, tree, gaps.>
The order in which the sequences appear in the output alignment.
-g -feature
Selects feature mode and specifies which features are to be used: e.g. all, maxplp, STRUCT, PFAM-A?
-same_feature_score
Score for aligning same features.
-diff_feature_score
Penalty for aligning different features.
-d -distance <wu, pair>
Distance method
-b -tree -guide-tree <nj, upgma>
Guide tree method.
-z -zcutoff
Parameter used in the wu-manber based distance calculation.
-i -in -input
Name of the input file.
-o -out -output
Name of the output file.
-a -gap_inc
Increases gap penalties depending on the number of existing gaps.
-f -format <fasta, msf, aln, clu, macsim>
The output format.
-q -quiet
Print nothing to STDERR. Read nothing from STDIN.
REFERENCES
o Timo Lassmann and Erik L.L. Sonnhammer (2005) Kalign - an accurate and fast multiple sequence alignment algorithm. BMC Bioinformatics
6:298
o Timo Lassmann, Oliver Frings and Erik L. L. Sonnhammer (2009) Kalign2: high-performance multiple alignment of protein and nucleotide
sequences allowing external features. Nucleic Acid Research 3:858?865.
AUTHORS
Timo Lassmann <timolassmann@gmail.com>
Upstream author of Kalign.
Charles Plessy <plessy@debian.org>
Wrote the manpage.
COPYRIGHT
Copyright (C) 2004, 2005, 2006, 2007, 2008 Timo Lassmann
Kalign is free software. You can redistribute it and/or modify it under the terms of the GNU General Public License as published by the
Free Software Foundation.
This manual page was written by Charles Plessy <plessy@debian.org> for the Debian(TM) system (but may be used by others). Permission is
granted to copy, distribute and/or modify this document under the same terms as kalign itself.
On Debian systems, the complete text of the GNU General Public License version 2 can be found in /usr/share/common-licenses/GPL-2.
kalign 2.04 February 25, 2009 KALIGN(1)