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Full Discussion: Help with a bash loop script
Top Forums UNIX for Beginners Questions & Answers Help with a bash loop script Post 303037350 by dre on Tuesday 30th of July 2019 09:34:25 AM
Old 07-30-2019
Help with a bash loop script

Create a single bash script that does the following:
a. Print out the number of occurrences for each motif that is found in the bacterial genome and output to a file called motif_count.txt
b. Create a fasta file for each motif (so 3 in total) which contains all of the genes and their corresponding sequences that have that motif. Each file should be named after the motif (ie ATG.txt) and outputted to a new directory called motifs

motif file is a txt file with these motifs : ATG, GGGGG, ATTTT
the bacterial genome file is fasta file with the following lines
Code:
 >gene1
GAAACTCGGTGTTGGCTTACCGGTCATTCCGAGCGTCATTTGGTTTTCGCGTCGTGGCGAAATGTGGTTCTACTACTCGTGGTGTATGCACTATTTATCCGGAATGTTCAGAGCGAGTAGACAATGGGTGCTCCACAATTGTGGCGGTCCCTAAGGGACTCACATATAGTGAGACACGCGTGAAATTCTGCTCACCACGTCCGAATCCGACAAATCATCTACTTCGACGGTA
>gene2
CGGAGATAAAGGACCCATACTGTACGACATTGTATTGCTCACCATGGTCAATCTTTGCGAGTTGTTGCAGCTCGCAGCTTCGTTCTGTCAATATAGCTTAGATACTGAGAAGAAGTTGCAGAGAAAGTCGCA

Moderator's Comments:
Mod Comment edit by bakunin: please use CODE-tags to let data, code and terminal output stand out. Thank you.

Last edited by bakunin; 07-30-2019 at 10:48 AM..
 

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TFBS::PatternGen::Gibbs(3pm)				User Contributed Perl Documentation			      TFBS::PatternGen::Gibbs(3pm)

NAME
TFBS::PatternGen::Gibbs - a pattern factory that uses Chip Lawrences Gibbs program SYNOPSIS
my $patterngen = TFBS::PatternGen::Gibbs->new(-seq_file=>'sequences.fa', -binary => '/Programs/Gibbs-1.0/bin/Gibbs' -nr_hits => 24, -motif_length => [8, 9, 10], -additional_params => '-x -r -e'); my $pfm = $patterngen->pattern(); # $pfm is now a TFBS::Matrix::PFM object DESCRIPTION
TFBS::PatternGen::Gibbs builds position frequency matrices using an advanced Gibbs sampling algorithm implemented in external Gibbs program by Chip Lawrence. The algorithm can produce multiple patterns from a single set of sequences. new Title : new Usage : my $db = TFBS::PatternGen::Gibbs->new(%args); Function: the constructor for the TFBS::PatternGen::Gibbs object Returns : a TFBS::PatternGen::Gibbs object Args : This method takes named arguments; you must specify one of the following three -seq_list # a reference to an array of strings # and/or Bio::Seq objects # or -seq_stream # A Bio::SeqIO object # or -seq_file # the name of the fasta file containing # all the sequences Other arguments are: -binary # a fully qualified path to Gibbs executable # OPTIONAL: default 'Gibbs' -nr_hits # a presumed number of pattern occurences in the # sequence set: it can be a single integer, e.g. # -nr_hits => 24 , or a reference to an array of # integers, e.g -nr_hits => [12, 24, 36] -motif_length # an expected length of motif in nucleotides: # it can be a single integer, e.g. # -motif_length => 8 , or a reference to an # array ofintegers, e.g -motif_length => [8..12] -additional_params # a string containing additional # command-line switches for the # Gibbs program pattern all_patterns patternSet The three methods listed above are used for the retrieval of patterns, and are common to all TFBS::PatternGen::* classes. Please see TFBS::PatternGen for details. perl v5.14.2 2008-01-24 TFBS::PatternGen::Gibbs(3pm)
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