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Full Discussion: Help with a bash loop script
Top Forums UNIX for Beginners Questions & Answers Help with a bash loop script Post 303037350 by dre on Tuesday 30th of July 2019 09:34:25 AM
Old 07-30-2019
Help with a bash loop script

Create a single bash script that does the following:
a. Print out the number of occurrences for each motif that is found in the bacterial genome and output to a file called motif_count.txt
b. Create a fasta file for each motif (so 3 in total) which contains all of the genes and their corresponding sequences that have that motif. Each file should be named after the motif (ie ATG.txt) and outputted to a new directory called motifs

motif file is a txt file with these motifs : ATG, GGGGG, ATTTT
the bacterial genome file is fasta file with the following lines
Code:
 >gene1
GAAACTCGGTGTTGGCTTACCGGTCATTCCGAGCGTCATTTGGTTTTCGCGTCGTGGCGAAATGTGGTTCTACTACTCGTGGTGTATGCACTATTTATCCGGAATGTTCAGAGCGAGTAGACAATGGGTGCTCCACAATTGTGGCGGTCCCTAAGGGACTCACATATAGTGAGACACGCGTGAAATTCTGCTCACCACGTCCGAATCCGACAAATCATCTACTTCGACGGTA
>gene2
CGGAGATAAAGGACCCATACTGTACGACATTGTATTGCTCACCATGGTCAATCTTTGCGAGTTGTTGCAGCTCGCAGCTTCGTTCTGTCAATATAGCTTAGATACTGAGAAGAAGTTGCAGAGAAAGTCGCA

Moderator's Comments:
Mod Comment edit by bakunin: please use CODE-tags to let data, code and terminal output stand out. Thank you.

Last edited by bakunin; 07-30-2019 at 10:48 AM..
 

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BP_REVTRANS-MOTIF(1p)					User Contributed Perl Documentation				     BP_REVTRANS-MOTIF(1p)

NAME
revtrans-motif - Reverse translate a Profam-like protein motif VERSION
Version 0.01 SYNOPSIS
From a file: revtrans-motif.pl -i motifs.txt Using pipes: revtrans-motif.pl < motifs.txt > output.txt Using interactively at the command prompt: $ revtrans-motif.pl MAAEEL[VIKP] 1. ATGGCNGCNGARGARYTNVHN [^P]H(IW){2,3} 2. NDNCAY(ATHTGG){2,3} DESCRIPTION
This script takes a protein motif as input and returns a degenerate oligonucleotide sequence corresponding to it. The main reason for doing this is to design degenerate primers that amplify a given sequence pattern. The input motif consists of a string of one-letter residues, with any of the following syntactic elements: [...] : Redundant position. A position in which more than one residue is allowed. Example: [TS]YW[RKSD] ^^ ^^^^ [^...] : Negated position. A position in which any residue is allowed, saved for those between brackets. Example: [^PW]MK[LAE] ^^ (...){n,m,...} : Repeated motif. A motif that is repeated n or m times. It can have any of the previous syntactic elements. Example: A[SN]C(TXX){2,4,8} ^^^ The allowed letters are those that correspond to the 20 natural aminoacids, plus: B = N + D Z = Q + E X = All OPTIONS
-i input-file: A file with a list of motifs to reverse translate. -h Display this help message. AUTHOR
Bruno Vecchi, "vecchi.b at gmail.com" BUGS
Please report any bugs or feature requests to "vecchi.b at gmail.com" COPYRIGHT &; LICENSE Copyright 2009 Bruno Vecchi, all rights reserved. This program is free software; you can redistribute it and/or modify it under the same terms as Perl itself. perl v5.14.2 2012-03-02 BP_REVTRANS-MOTIF(1p)
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