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Top Forums UNIX for Beginners Questions & Answers How to append two fasta files? Post 303035986 by yifangt on Tuesday 11th of June 2019 02:32:15 PM
Old 06-11-2019
combine and upgrade by the second fasta file

I put my answer back as I met the scenarios:
1) when file2.fasta contains more entries than in file1.fasta and vice versa. and
2) when the sequence part can have more than one row;
Code:
awk 'BEGIN{RS=">";FS="\n"} {A[$1]=$2} END{for (i in A) {if (i) print ">"i,FS,A[i]}}' file1.fasta file2.fasta

This only works for 2-line fasta sequence files (i.e. each entry has two lines, One starts with ">" as the header, the other is the DNA sequence. @vgersh99, could you please elaborate your code for scenario 2)? Thanks!
Code:
file1.fasta
>Contig_1:90600-91187
GACCGTCATCAATTCCTGTTCCTTGCCCTTGACGACCTCATCCACGTCCTTGATGGCCTT 
>Contig_24:26615-28387
TTCGCCGCGCTCCAAACGGGCGATCTCCTCGGCGCGGGCCGCCAGGATCAGCGCCG
>Contig_98:35323-35886
GACGAAGCGCTCGCCAAGGCCGAAGAAGAAGGCCTGGATCTGGTCGAAATCCAGCCGCAG               
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGA

file2.fasta:
>Contig_1:90600-91187 
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGAACAGGAATTGATGACGGTC
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGAACAGG
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGA
>Contig_24:26615-28387
GCTGCGGCGCTGATCCTGGCGGCCCGCGCCGAGGAGATCGCCCGTTTGGAGCGCGGCGAA

output:
>Contig_1:90600-91187 
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGAACAGGAATTGATGACGGTC
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGAACAGG
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGA
>Contig_24:26615-28387
GCTGCGGCGCTGATCCTGGCGGCCCGCGCCGAGGAGATCGCCCGTTTGGAGCGCGGCGAA
>Contig_98:35323-35886
GACGAAGCGCTCGCCAAGGCCGAAGAAGAAGGCCTGGATCTGGTCGAAATCCAGCCGCAG               
AAGGCCATCAAGGACGTGGATGAGGTCGTCAAGGGCAAGGA

This User Gave Thanks to yifangt For This Post:
 

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Bio::SeqIO::tab(3pm)					User Contributed Perl Documentation				      Bio::SeqIO::tab(3pm)

NAME
Bio::SeqIO::tab - nearly raw sequence file input/output stream. Reads/writes id" "sequence" " SYNOPSIS
Do not use this module directly. Use it via the Bio::SeqIO class. DESCRIPTION
This object can transform Bio::Seq objects to and from tabbed flat file databases. It is very useful when doing large scale stuff using the Unix command line utilities (grep, sort, awk, sed, split, you name it). Imagine that you have a format converter 'seqconvert' along the following lines: my $in = Bio::SeqIO->newFh(-fh => *STDIN , '-format' => $from); my $out = Bio::SeqIO->newFh(-fh=> *STDOUT, '-format' => $to); print $out $_ while <$in>; then you can very easily filter sequence files for duplicates as: $ seqconvert < foo.fa -from fasta -to tab | sort -u | seqconvert -from tab -to fasta > foo-unique.fa Or grep [-v] for certain sequences with: $ seqconvert < foo.fa -from fasta -to tab | grep -v '^S[a-z]*control' | seqconvert -from tab -to fasta > foo-without-controls.fa Or chop up a huge file with sequences into smaller chunks with: $ seqconvert < all.fa -from fasta -to tab | split -l 10 - chunk- $ for i in chunk-*; do seqconvert -from tab -to fasta < $i > $i.fa; done # (this creates files chunk-aa.fa, chunk-ab.fa, ..., each containing 10 # sequences) FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHORS
Philip Lijnzaad, p.lijnzaad@med.uu.nl APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ next_seq Title : next_seq Usage : $seq = $stream->next_seq() Function: returns the next sequence in the stream Returns : Bio::Seq object Args : write_seq Title : write_seq Usage : $stream->write_seq($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Bio::Seq object perl v5.14.2 2012-03-02 Bio::SeqIO::tab(3pm)
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