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Top Forums UNIX for Beginners Questions & Answers How to count the length of fasta sequences? Post 303033663 by dineshkumarsrk on Wednesday 10th of April 2019 05:43:26 AM
Old 04-10-2019
is it possible to print the order as same as id.txt. The command works fine, but the order has been changed. For example, id.txt file listed the headers as follows,
Code:
seq1
seq2

Where as my unique.fasta file has the sequences in the following order,
Code:
>seq2
ATGCTTA
>seq1
GCTAGT

But, the command print seq2 length first followed by seq1 as shown below.
Code:
seq2     7
seq1      6

Therefore, is it possible to keep the order as same as id.txt.

--- Post updated at 09:43 AM ---

Dear singh,
Is it possible to print the order as same as id.txt. The command works fine, but the order has been changed. For example, id.txt file listed the headers as follows,
Code:
seq1
seq2

Whereas, my unique.fasta file has the sequences in the following order,
Code:
>seq2
ATGCTTA
>seq1
GCTAGT

But, the command print seq2 length first followed by seq1 as shown below.
Code:
seq2     7
seq1    6

Therefore, is it possible to keep the order as same as id.txt.
 

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VERSION:(1)							   User Commands						       VERSION:(1)

NAME
PyNAST - alignment of short DNA sequences SYNOPSIS
pynast [options] {-i input_fp -t template_fp} DESCRIPTION
[] indicates optional input (order unimportant) {} indicates required input (order unimportant) Example usage: pynast -i my_input.fasta -t my_template.fasta OPTIONS
--version show program's version number and exit -h, --help show this help message and exit -t TEMPLATE_FP, --template_fp=TEMPLATE_FP path to template alignment file [REQUIRED] -i INPUT_FP, --input_fp=INPUT_FP path to input fasta file [REQUIRED] -v, --verbose Print status and other information during execution [default: False] -p MIN_PCT_ID, --min_pct_id=MIN_PCT_ID minimum percent sequence identity to consider a sequence a match [default: 75.0] -l MIN_LEN, --min_len=MIN_LEN minimum sequence length to include in NAST alignment [default: 1000] -m PAIRWISE_ALIGNMENT_METHOD, --pairwise_alignment_method=PAIRWISE_ALIGNMENT_METHOD method for performing pairwise alignment [default: uclust] -a FASTA_OUT_FP, --fasta_out_fp=FASTA_OUT_FP path to store resulting alignment file [default: derived from input filepath] -g LOG_FP, --log_fp=LOG_FP path to store log file [default: derived from input filepath] -f FAILURE_FP, --failure_fp=FAILURE_FP path to store file of seqs which fail to align [default: derived from input filepath] -e MAX_E_VALUE, --max_e_value=MAX_E_VALUE Depreciated. Will be removed in PyNAST 1.2 -d BLAST_DB, --blast_db=BLAST_DB Depreciated. Will be removed in PyNAST 1.2 SEE ALSO
http://pynast.sourceforge.net Version: pynast 1.1 August 2011 VERSION:(1)
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