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Top Forums Shell Programming and Scripting awk to change value in field according to another Post 303027092 by bakunin on Sunday 9th of December 2018 05:52:03 AM
Old 12-09-2018
Quote:
Originally Posted by Don Cragun
Note that I am not sure why bakunin suggested using \> in the exon.sh command. Doing that makes the redirection operator and the intended output file become operands to exon.sh instead of being a redirection.
Exactly this was the point. When i suggested debugging the command i suggested to use an echo command in front of it. This echo would have been redirected instead of displayed. This was why i suggested to escape the redirection to display - for debugging purposes - the commands which would be issued by the script.

Quote:
The last thing, if the variables do produce the correct values, is to test the command itself: instead of running it we just display it. Notice that we need to escape the redirection:
[...]
Code:
echo /path/to/exon.sh static "$file" \> "path/to/${pref}_output.txt"

Of course the escaped redirection has to be reverted back to normal once the debugging is over.

bakunin

Last edited by bakunin; 12-09-2018 at 06:59 AM..
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Bio::Tools::Sim4::Exon(3pm)				User Contributed Perl Documentation			       Bio::Tools::Sim4::Exon(3pm)

NAME
Bio::Tools::Sim4::Exon - A single exon determined by an alignment SYNOPSIS
# See Bio::Tools::Sim4::Results for a description of the context. # an instance of this class is-a Bio::SeqFeature::SimilarityPair # coordinates of the exon (recommended way): print "exon from ", $exon->start(), " to ", $exon->end(), " "; # the same (feature1() inherited from Bio::SeqFeature::FeaturePair) print "exon from ", $exon->feature1()->start(), " to ", $exon->feature1()->end(), " "; # also the same (query() inherited from Bio::SeqFeature::SimilarityPair): print "exon from ", $exon->query()->start(), " to ", $exon->query()->end(), " "; # coordinates on the matching EST (recommended way): print "matches on EST from ", $exon->est_hit()->start(), " to ", $exon->est_hit()->end(), " "; # the same (feature2() inherited from Bio::SeqFeature::FeaturePair) print "matches on EST from ", $exon->feature2()->start(), " to ", $exon->feature2()->end(), " "; # also the same (subject() inherited from Bio::SeqFeature::SimilarityPair): print "exon from ", $exon->subject()->start(), " to ", $exon->subject()->end(), " "; DESCRIPTION
This class inherits from Bio::SeqFeature::SimilarityPair and represents an exon on a genomic sequence determined by similarity, that is, by aligning an EST sequence (using Sim4 in this case). Consequently, the notion of query and subject is always from the perspective of the genomic sequence: query refers to the genomic seq, subject to the aligned EST hit. Because of this, $exon->start(), $exon->end() etc will always return what you expect. To get the coordinates on the matching EST, refer to the properties of the feature returned by est_hit(). FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHOR - Ewan Birney, Hilmar Lapp Ewan Birney <birney-at-sanger.ac.uk> Hilmar Lapp <hlapp-at-gmx.net> or <hilmar.lapp-at-pharma.novartis.com>. APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ percentage_id Title : percentage_id Usage : $obj->percentage_id($newval) Function: This is a synonym for 100 * $obj->est_hit()->frac_identical(). Returns : value of percentage_id Args : newvalue (optional) est_hit Title : est_hit Usage : $est_feature = $obj->est_hit(); Function: Returns the EST hit pointing to (i.e., aligned to by Sim4) this exon (i.e., genomic region). At present, merely a synonym for $obj->feature2(). Returns : An Bio::SeqFeatureI implementing object. Args : perl v5.14.2 2012-03-02 Bio::Tools::Sim4::Exon(3pm)
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