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Top Forums Shell Programming and Scripting Retrieving sequences corresponding to start and end position Post 303023854 by Ibk on Monday 24th of September 2018 05:23:14 PM
Old 09-24-2018
Hi,

After trying to retrieve the sequence using

Code:
awk 'NR == 1 {print "seq_id Start End Nul_Seq"} NR == FNR {SEQ = SEQ $0;next}{if ($2>$3) print $0, substr (SEQ, $3, $2-$3+1); else print $0, substr (SEQ, $2, $3-$2+1)}' my_seq.fasta file_2.txt > output.txt

I have a sequence NC_004064.1 (from NCBI) in a file and another file with Query ID, start position and end position

Code:
>my_querry_seq.fasta 
GTGATTTAATTATAGAGAGATAGTGACTTTCACTTTTCTTTCTTTGCATTTCGCTGGACTCAAACTGTTC
CATAATGGCTCCAGTTGTTTCCAGAGATCAATGTAAACCTAAAACACCAAAGCCCCACCGACCAGCCCCT
CCCCACAGGTGCACTACCAGGTGCCCTGAGGATTGTGGGTGGTACGTGGGTCGCTGCTCCTGCCCCAACG
TCTGCCAACGCGAGGGTTGGGACGACTTCTTCGTAGCTGACAAGGTGAAGCCTCCTAGCTACGTCGCCTC
AAAGACCTCGGTCGCAGATGTTGTCGACTGGCTACTTGAAGAAGACCCTGCTACGGACGGCCCCTCGGAA
TTTGACCTCACGCAATTTTTCCAGGCTTACACGGACAAGTCCCACCAGATCCACCGTGACTATGCCCCGG
ACCAGCTTGCTCAGGCCCTTGACATGGCTTACATACTATCGGTGGACCCCCCGGACATCAAGCTCCCAGA
GTATGAGGCAACTAGGTTCACCCATGACACCTCCTACAAGGGCAAACTGCCTAAGTGGCTGCGTGTGTAT
GGCATCAAGTCCCGAGAGCTAGCGAAGAAGGCTGTAACCAACATTCGGGGTGGAGCCCATTGGGCCAAGG
GGCTGTTCAAGCAAATGTGGGACTCGTTGCCTGGTTGGAGTGAGGTGGAGGCGTACTTCAAAGCTTTCTT
CGCTGGCATCATCACCGGTGTTGAGGACGCGCTGTCGAAGTCTCCGTCATCGGTGTGGACCTCACTCAAG
CTCACGCCGTTGCTATACATATGGCGCAACATCAACGAGTGCTCTGATATTGCTGTGATACTAGGAGCCT
TCTGGGCGACACTGGAACTTTACAACATCCCATCCAAAGTGTACGACCTGGTGTCAACTGCACTTGGCCC


file_2.txt

Code:
ID start end
my_seq 10 40
my_seq 35 65
my_seq 50 20
my_seq 76 96


I want to use the second file to retrieve the sequence from the start to end position printed in front of each

Note that in line 3 the start (50) position is greater than the end (20) but want this to print from 50 to 20

I want the sequence printed in front in this format

Expected Output
ID Start End Sequence


I will really appreciate your help.

Thanks

Last edited by vgersh99; 09-24-2018 at 06:51 PM.. Reason: please start using code tags!
 

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Bio::Index::Fasta(3pm)					User Contributed Perl Documentation				    Bio::Index::Fasta(3pm)

NAME
Bio::Index::Fasta - Interface for indexing (multiple) fasta files SYNOPSIS
# Make an index for one or more fasta files use Bio::Index::Fasta; use strict; my $Index_File_Name = shift; my $inx = Bio::Index::Fasta->new(-filename => $Index_File_Name, -write_flag => 1); $inx->make_index(@ARGV); # Once the index is made it can accessed, either in the # same script or a different one use Bio::Index::Fasta; use strict; my $Index_File_Name = shift; my $inx = Bio::Index::Fasta->new(-filename => $Index_File_Name); my $out = Bio::SeqIO->new(-format => 'Fasta', -fh => *STDOUT); foreach my $id (@ARGV) { my $seq = $inx->fetch($id); # Returns Bio::Seq object $out->write_seq($seq); } # or, alternatively my $id; my $seq = $inx->get_Seq_by_id($id); # identical to fetch() DESCRIPTION
Inherits functions for managing dbm files from Bio::Index::Abstract.pm, and provides the basic funtionallity for indexing fasta files, and retrieving the sequence from them. For best results 'use strict'. Bio::Index::Fasta supports the Bio::DB::BioSeqI interface, meaning it can be used as a Sequence database for other parts of bioperl Additional example code is available in scripts/index/*PLS and in the Bioperl Tutorial (<http://www.bioperl.org/wiki/Bptutorial.pl>) Note that by default the key for the sequence will be the first continuous string after the '>' in the fasta header. If you want to use a specific substring of the fasta header you must use the id_parser() method. You can also set or customize the unique key used to retrieve by writing your own function and calling the id_parser() method. For example: $inx->id_parser(&get_id); # make the index $inx->make_index($file_name); # here is where the retrieval key is specified sub get_id { my $line = shift; $line =~ /^>.+gi|(d+)/; $1; } FEED_BACK Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHOR - James Gilbert Email - jgrg@sanger.ac.uk APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ _file_format Title : _file_format Function: The file format for this package, which is needed by the SeqIO system when reading the sequence. Returns : 'Fasta' _index_file Title : _index_file Usage : $index->_index_file( $file_name, $i ) Function: Specialist function to index FASTA format files. Is provided with a filename and an integer by make_index in its SUPER class. Example : Returns : Args : id_parser Title : id_parser Usage : $index->id_parser( CODE ) Function: Stores or returns the code used by record_id to parse the ID for record from a string. Useful for (for instance) specifying a different parser for different flavours of FASTA file. Returns &default_id_parser (see below) if not set. If you supply your own id_parser subroutine, then it should expect a fasta description line. An entry will be added to the index for each string in the list returned. Example : $index->id_parser( &my_id_parser ) Returns : ref to CODE if called without arguments Args : CODE default_id_parser Title : default_id_parser Usage : $id = default_id_parser( $header ) Function: The default Fasta ID parser for Fasta.pm Returns $1 from applying the regexp /^>s*(S+)/ to $header. Returns : ID string Args : a fasta header line string perl v5.14.2 2012-03-02 Bio::Index::Fasta(3pm)
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