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Top Forums Shell Programming and Scripting Perl to adjust coordinates based on repeat string Post 303021949 by cmccabe on Tuesday 21st of August 2018 03:17:02 PM
Old 08-21-2018
Thank you very much, works great.... very nice Smilie.

------ Post updated at 02:17 PM ------

Looking into the data there are a couple of modifications that I am unsure how to incorporate. The streches are detected and the loop is amazing and very helpful. The math is slightly different and the repeat stretch is opposite, hopefully the examples will help to show but if strand=- then the bases in blue (rightmost) are the start or first number after the : and the repeats in green are opposite:

Code:
that is:
A or a is T or t
T or t is A or a
C or c is G or g
G or g is C or c

Thank you very much Smilie.

input
Code:
>hg19_ncbiRefSeq_SCN1A range=chr2:166905371-166905484 5'pad=25 3'pad=25 strand=- repeatMasking=none
catacgactttcttttttcaaacagGATATCATTATTTCCTGGAGGGTTT
TTTAGATGCACTACTATGTGGAAATAGCTCTGATGCAGGgtaagtcaata
atttgtgtgtatct
>hg19_ncbiRefSeq_SCN1A range=chr2:166895908-166896131 5'pad=25 3'pad=25 strand=- repeatMasking=none
tagattaattttgttttgatcttagGTTTTCACTGGGATCTTTACAGCAG
AAATGTTTCTGAAAATTATTGCCATGGATCCTTACTATTATTTCCAAGAA
GGCTGGAATATCTTTGACGGTTTTATTGTGACGCTTAGCCTGGTAGAACT
TGGACTCGCCAATGTGGAAGGATTATCTGTTCTCCGTTCATTTCGATTGg
taaaaaaaaaaaaaaaaagcacca
>hg19_ncbiRefSeq_SCN2A range=chr2:166168510-166168623 5'pad=25 3'pad=25 strand=+ repeatMasking=none
TTTTTT range=chr2:166168546-166168551
>hg19_ncbiRefSeq_SLC2A1 range=chr1:43393251-43393504 5'pad=25 3'pad=25 strand=- repeatMasking=none
No Homopolymers Detected

desired output
Code:
>hg19_ncbiRefSeq_SCN1A range=chr2:166905371-166905484 5'pad=25 3'pad=25 strand=- repeatMasking=none
AAAAAA chr2:166905432-166905437
aaaaaa chr2:166905467-166905472
>hg19_ncbiRefSeq_SCN1A range=chr2:166895908-166896131 5'pad=25 3'pad=25 strand=- repeatMasking=none
ttttttttttttttt range=chr2:166896110-166896124
tttttttttttttttt range=chr2:166896110-166896125
ttttttttttttttttt range=chr2:166896110-166896126
>hg19_ncbiRefSeq_SCN2A range=chr2:166168510-166168623 5'pad=25 3'pad=25 strand=+ repeatMasking=none
TTTTTT range=chr2:166168545-166168550
>hg19_ncbiRefSeq_SLC2A1 range=chr1:43393251-43393504 5'pad=25 3'pad=25 strand=- repeatMasking=none
Nothing Detected


In the line with > if the strand=+ then everything is good except that the calculated range=chr2:166170446-166170451 is 1 digit off. An example is

Code:
>hg19_ncbiRefSeq_SCN2A range=chr2:166168510-166168623 5'pad=25 3'pad=25 strand=+ repeatMasking=none
TTTTTT range=chr2:166168546-166168551

is

Code:
>hg19_ncbiRefSeq_SCN2A range=chr2:166168510-166168623 5'pad=25 3'pad=25 strand=+ repeatMasking=none
TTTTTT range=chr2:166168545-166168550

In the line with > if the strand=- then the math is slightly different in that the first # is really the end position. An example is,

Code:
>hg19_ncbiRefSeq_SCN1A range=chr2:166905371-166905484 5'pad=25 3'pad=25 strand=- repeatMasking=none
catacgactttcttttttcaaacagGATATCATTATTTCCTGGAGGGTTT
TTTAGATGCACTACTATGTGGAAATAGCTCTGATGCAGGgtaagtcaata
atttgtgtgtatct

is

Code:
>hg19_ncbiRefSeq_SCN1A range=chr2:166905371-166905484 5'pad=25 3'pad=25 strand=- repeatMasking=none
AAAAAA chr2:166905432-166905437
aaaaaa chr2:166905467-166905472

If Nothing Detected then the line is as is. Thank you again Smilie.

Last edited by cmccabe; 08-21-2018 at 05:05 PM.. Reason: added details
 

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