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Top Forums Shell Programming and Scripting Perl to adjust coordinates based on repeat string Post 303021850 by cmccabe on Saturday 18th of August 2018 12:28:12 PM
Old 08-18-2018
Perl to adjust coordinates based on repeat string

In the file below I am trying to count the given repeats of A,T,C,G in each string of letters. Each sequence is below the > and it is possible for a string of repeats to wrap from the line above. For example, in the first line the last letter is a T and the next lines has 3 more. I think the below would work, but I am also trying to count the position range of the repeat using the range=, where the first # represents the leftmost (in the first line that is aaa) and the second # rightmost (in the first line that is taa). So using the 4T as an example the output is in the example output.

The t{4} is the repeat that can change, for example if I am after 7g then that would be g{7}... the lower case letters in the sequence are counted along with the capital letters if they satisfy the criteria. I think both would be captured in the order there are seen, as that is important to know. For example, 4t occurs at chr2:166911127-166911130... even though there are 6t in that strech only the 4t satisfy the criteria and are counted. An example output is in the output for two sequences. Thank you Smilie.

file
Code:
>hg19_ncbiRefSeq_Gene range=chr2:166911123-166911301 5'pad=25 3'pad=25 strand=- repeatMasking=none
aaattttttggatgcttgttttcagATACACCTTCACAGGAATATATACT
TTTGAATCACTTATAAAAATTATTGCAAGGGGATTCTGTTTAGAAGATTT
TACTTTCCTTCGGGATCCATGGAACTGGCTCGATTTCACTGTCATTACAT
TTGCgtaagtgccttttttgaaactttaa
>hg19_ncbiRefSeq_Gene range=chr2:166909337-166909478 5'pad=25 3'pad=25 strand=- repeatMasking=none
tttgtgtgtgaactccctattacagGTACGTCACAGAGTTTGTGGACCTG
GGCAATGTCTCGGCATTGAGAACATTCAGAGTTCTCCGAGCATTGAAGAC

example output
Code:
TTTT chr2:166911173-166911176

description
Code:
the first T is 50 in so that is added to the 166911123 and that is the new value after the : and the last T is 53 so that is added to the 166911123 and that is the new value after the -.

perl
Code:
perl -076 -nE 'chomp; s/(.+)// && say qq{>$1}; s/\s//g; say $1 while /(t{4})/gi' file

output for two sequences
Code:
tttt chr2=166911127-166911130
TTTT chr2:166911173-166911176


Last edited by cmccabe; 08-18-2018 at 01:33 PM.. Reason: fixed format
 

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