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Full Discussion: Sequence extraction
Top Forums Shell Programming and Scripting Sequence extraction Post 302951322 by harpreetmanku04 on Wednesday 5th of August 2015 06:21:37 AM
Old 08-05-2015
may be that entries would be at the bottom of the file actually sir, i just copied and posted very few entries from file1 and file2 may be matched would be skipped. i cant paste my whole data file here i have 4565005 sequence entries in my data.

and about negative value, please provide me the script for like 300 250 = 50
no problem if there is 250-300 =-50 i will correct it and will then apply script.
 

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Ace::Sequence::Homol(3pm)				User Contributed Perl Documentation				 Ace::Sequence::Homol(3pm)

NAME
Ace::Sequence::Homol - Temporary Sequence Homology Class SYNOPSIS
# Get all similarity features from an Ace::Sequence @homol = $seq->features('Similarity'); # sort by score @sorted = sort { $a->score <=> $b->score } @homol; # the last one has the highest score $best = $sorted[$#sorted]; # fetch its associated Ace::Sequence::Homol $homol = $best->target; # print out the sequence name, DNA, start and end print $homol->name,' ',$homol->start,'-',$homol->end," "; print $homol->asDNA; DESCRIPTION
Ace::Sequence::Homol is a subclass of Ace::Object (not Ace::Sequence) which is specialized for returning information about a DNA or protein homology. This is a temporary placeholder for a more sophisticated homology class which will include support for alignments. OBJECT CREATION
You will not ordinarily create an Ace::Sequence::Homol object directly. Instead, objects will be created in response to an info() or group() method call on a similarity feature in an Ace::Sequence::Feature object. If you wish to create an Ace::Sequence::Homol object directly, please consult the source code for the new() method. OBJECT METHODS
Most methods are inherited from Ace::Object. The following methods are also supported: start() $start = $homol->start; Returns the start of the area that is similar to the Ace::Sequence::Feature from which his homology was derived. Coordinates are relative to the target homology. end() $end = $homol->end; Returns the end of the area that is similar to the Ace::Sequence::Feature from which his homology was derived. Coordinates are relative to the target homology. asString() $label = $homol->asString; Returns a human-readable identifier describing the nature of the feature. The format is: $name/$start-$end for example: HUMGEN13/1-67 This method is also called automatically when the object is treated in a string context. SEE ALSO
Ace, Ace::Object, Ace::Sequence,Ace::Sequence::FeatureList, Ace::Sequence::Feature, GFF AUTHOR
Lincoln Stein <lstein@w3.org> with extensive help from Jean Thierry-Mieg <mieg@kaa.crbm.cnrs-mop.fr> Copyright (c) 1999, Lincoln D. Stein This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself. See DISCLAIMER.txt for disclaimers of warranty. perl v5.14.2 2001-09-17 Ace::Sequence::Homol(3pm)
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