11-06-2014
You may want to write a Perl wrapper with the YAML library. Perl and YAML are really good at reading configuration files. You would then have a configuration file with all of the parameters that you need to make the bowtie2 call, you can parse all of the inputs and more simply change the inputs to what you need and make it work. It would probably be easier to read and understand then the approach you are taking. etc..etc..
Here is the install page for YAML. I am using Perl and YAML to build a software installer. The customer will only need to provide the response file to make it work. Which is much easier than handling each parameter separately. And I can directly reference any parameter as $yaml_hash->{field_name}.
YAML - search.cpan.org
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LEARN ABOUT DEBIAN
bowtie2-inspect
BOWTIE2-INSPECT(1) User Commands BOWTIE2-INSPECT(1)
NAME
bowtie2-inspect - extracts information from a bowtie2 index
SYNOPSIS
bowtie2-inspect [options]* <bt2_base>
DESCRIPTION
Bowtie 2 version 2.0.0-beta6 by Ben Langmead (blangmea@jhsph.edu)
<bt2_base>
bt2 filename minus trailing .1.bt2/.2.bt2
By default, prints FASTA records of the indexed nucleotide sequences to standard out. With -n, just prints names. With -s, just
prints a summary of the index parameters and sequences. With -e, preserves colors if applicable.
OPTIONS
-a/--across <int>
Number of characters across in FASTA output (default: 60)
-n/--names
Print reference sequence names only
-s/--summary
Print summary incl. ref names, lengths, index properties
-e/--bt2-ref
Reconstruct reference from .bt2 (slow, preserves colors)
-v/--verbose
Verbose output (for debugging)
-h/--help
print detailed description of tool and its options
--help print this usage message
64-bit Built on lxcsid Fri Jun 1 20:13:13 UTC 2012 Compiler: gcc version 4.7.0 (Debian 4.7.0-8) Options: -O3 -m64 -msse2 -funroll-loops
-g3 Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8}
bowtie2-inspect version 2.0.0-beta6 June 2012 BOWTIE2-INSPECT(1)