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Full Discussion: Parsing the test file
Top Forums Shell Programming and Scripting Parsing the test file Post 302901368 by Corona688 on Tuesday 13th of May 2014 01:59:11 PM
Old 05-13-2014
I'm not quite sure what you want. 'On the basis of' doesn't tell us what basis, just that there is one involving certain columns. "unique count" in particular doesn't make sense -- the counts in your resulting output are not all unique.

Do you mean that if, for a particular refgene, if there are more than one with the same count, you want to keep the one with the lowest or highest tss depending on the last column +/- ?
 

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Ace::Sequence::Transcript(3pm)				User Contributed Perl Documentation			    Ace::Sequence::Transcript(3pm)

NAME
Ace::Sequence::Transcript - Simple "Gene" Object SYNOPSIS
# open database connection and get an Ace::Object sequence use Ace::Sequence; # get a megabase from the middle of chromosome I $seq = Ace::Sequence->new(-name => 'CHROMOSOME_I, -db => $db, -offset => 3_000_000, -length => 1_000_000); # get all the transcripts @genes = $seq->transcripts; # get the exons from the first one @exons = $genes[0]->exons; # get the introns @introns = $genes[0]->introns # get the CDSs (NOT IMPLEMENTED YET!) @cds = $genes[0]->cds; DESCRIPTION
Ace::Sequence::Gene is a subclass of Ace::Sequence::Feature. It inherits all the methods of Ace::Sequence::Feature, but adds the ability to retrieve the annotated introns and exons of the gene. OBJECT CREATION
You will not ordinarily create an Ace::Sequence::Gene object directly. Instead, objects will be created in response to a transcripts() call to an Ace::Sequence object. OBJECT METHODS
Most methods are inherited from Ace::Sequence::Feature. The following methods are also supported: exons() @exons = $gene->exons; Return a list of Ace::Sequence::Feature objects corresponding to annotated exons. introns() @introns = $gene->introns; Return a list of Ace::Sequence::Feature objects corresponding to annotated introns. cds() @cds = $gene->cds; Return a list of Ace::Sequence::Feature objects corresponding to coding sequence. THIS IS NOT YET IMPLEMENTED. relative() $relative = $gene->relative; $gene->relative(1); This turns on and off relative coordinates. By default, the exons and intron features will be returned in the coordinate system used by the gene. If relative() is set to a true value, then coordinates will be expressed as relative to the start of the gene. The first exon will (usually) be 1. SEE ALSO
Ace, Ace::Object, Ace::Sequence,Ace::Sequence::Homol, Ace::Sequence::Feature, Ace::Sequence::FeatureList, GFF AUTHOR
Lincoln Stein <lstein@cshl.org> with extensive help from Jean Thierry-Mieg <mieg@kaa.crbm.cnrs-mop.fr> Copyright (c) 1999, Lincoln D. Stein This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself. See DISCLAIMER.txt for disclaimers of warranty. POD ERRORS
Hey! The above document had some coding errors, which are explained below: Around line 168: You forgot a '=back' before '=head1' perl v5.14.2 2001-05-22 Ace::Sequence::Transcript(3pm)
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