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Top Forums Shell Programming and Scripting Eliminating sequences based on Distances Post 302813233 by jim mcnamara on Sunday 26th of May 2013 09:19:35 PM
Old 05-26-2013
None of the core UNIX utilities can read an xls file. It is purely a a windows-ism. A comma-delimited file, sure UNIX can read it. You need to convert your example to a csv (or tab-delimited), or just post a copy of the csv, or tab-delimited file.
 

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Dotur(1)						      General Commands Manual							  Dotur(1)

NAME
dotur - A program for calculating descriptive statistics for sequence libraries SYNOPSIS
dotur [-i Iterations (<1000>)] [-c ClusterMethod (<f>, n, a)] [-p Precision (10, <100>, 1000, 10000)] [-l] [-j] <file> OPTIONS
-i: Number of iterations (default = 1000) -c: Clustering method - (f) furthest neighbor, (n) nearest neighbor, (a) average neighbor (default = f) -p: Precision of distances for output, increasing can dramatically lengthen execution times - 10, 100, 1000, 10000 (default = 100) -l: Input file is lower triangular (default = square matrix) -r: Calculates rarefaction curves for each parameter, can dramatically lengthen execution times. Simple rarefaction curve always calcu- lated. -stop: Stops clustering when cutoff has been reached. -wrep: Samples with replacement. -jumble: Jumble the order of the distance matrix. -sim: Converts similarity score to distance (D=1-S). DESCRIPTION
This is the Debian GNU/Linux version of dotur. DOTUR is a computer program that takes a distance matrix describing the genetic distance between DNA sequence data and assigns sequences to operational taxonomic units (OTUs) using either the furthest, average, or nearest neighbor algorithms for all possible distances that can be described using the distance matrix. Using the OTU composition data, DOTUR constructs collector's and rarefaction curves for sampling intensity, richness estimators, and diversity indices. AUTHOR
This manual page was compiled from the package description and the output of help2man by Thorsten Alteholz <debian@alteholz.de>, for the Debian GNU/Linux system (but may be used by others). DOTUR
June 03, 2011 Dotur(1)
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