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Top Forums Shell Programming and Scripting Perl open(CMD, "cmd |"); buffering problem.. Post 302593563 by thegeek on Friday 27th of January 2012 10:01:18 AM
Old 01-27-2012
Are you sure the 3rd party application writes everything to STDOUT ? Bcoz, you are likely to miss the stuffs printed on STDERR ... But when you execute manually and see output you are likely to miss where it prints .. So try doing with redirection of error to different place & confirm !
 

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FASTQ_QUALITY_TRIMMER(1)					   User Commands					  FASTQ_QUALITY_TRIMMER(1)

NAME
fastq_quality_trimmer - Filters sequences based on quality DESCRIPTION
usage: fastq_quality_trimmer [-h] [-v] [-t N] [-l N] [-z] [-i INFILE] [-o OUTFILE] Part of FASTX Toolkit 0.0.13.1 by A. Gordon (gor- don@cshl.edu) [-h] = This helpful help screen. [-t N] = Quality threshold - nucleotides with lower quality will be trimmed (from the end of the sequence). [-l N] = Minimum length - sequences shorter than this (after trimming) will be discarded. Default = 0 = no minimum length. [-z] = Compress output with GZIP. [-i INFILE] = FASTQ input file. default is STDIN. [-o OUTFILE] = FASTQ output file. default is STDOUT. [-v] = Verbose - report number of sequences. If [-o] is specified, report will be printed to STDOUT. If [-o] is not specified (and output goes to STDOUT), report will be printed to STDERR. SEE ALSO
The quality of this automatically generated manpage might be insufficient. It is suggested to visit http://hannonlab.cshl.edu/fastx_toolkit/commandline.html to get a better layout as well as an overview about connected FASTX tools. fastq_quality_trimmer 0.0.13.1 April 2012 FASTQ_QUALITY_TRIMMER(1)
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