Hi
I have an alignment file (.fasta) with ~80 sequences. They look like this-
>JV101.contig00066(+):25302-42404|sequence_index=0|block_index=4|species=JV101|JV101_4_0
GAGGTTAATTATCGATAACGTTTAATTAAAGTGTTTAGGTGTCATAATTT
TAAATGACGATTTCTCATTACCATACACCTAAATTATCATCAATCTGAAT... (2 Replies)
I have fasta files with multiple sequences in each. I need to change the sequence name headers from:
>accD:_59176-60699
ATGGAAAAGTGGAGGATTTATTCGTTTCAGAAGGAGTTCGAACGCA
>atpA_(reverse_strand):_showing_revcomp_of_10525-12048
ATGGTAACCATTCAAGCCGACGAAATTAGTAATCTTATCCGGGAAC... (2 Replies)
Hi,
I want to match the sequence id (sub-string of line starting with '>' and extract the information upto next '>' line ). Please help .
input
> fefrwefrwef X900
AGAGGGAATTGG
AGGGGCCTGGAG
GGTTCTCTTC
> fefrwefrwef X932
AGAGGGAATTGG
AGGAGGTGGAG
GGTTCTCTTC
> fefrwefrwef X937... (2 Replies)
I have two files. File1 is shown below.
>153L:B|PDBID|CHAIN|SEQUENCE
RTDCYGNVNRIDTTGASCKTAKPEGLSYCGVSASKKIAERDLQAMDRYKTIIKKVGEKLCVEPAVIAGIISRESHAGKVL
KNGWGDRGNGFGLMQVDKRSHKPQGTWNGEVHITQGTTILINFIKTIQKKFPSWTKDQQLKGGISAYNAGAGNVRSYARM
DIGTTHDDYANDVVARAQYYKQHGY
>16VP:A|PDBID|CHAIN|SEQUENCE... (7 Replies)
Please provide script/commands to convert text file to HTML tabular format.
No need of styles and colours, just output and a heading in table is required.
Output file will be send via email and will be seen from outlook.
(script required without using awk).
output file content: (sar... (7 Replies)
Hi
How can I extract sequences from a fasta file with respect a certain criteria? The beginning of my file (containing in total more than 1000 sequences) looks like this:
>H8V34IS02I59VP
SDACNDLTIALLQIAREVRVCNPTFSFRWHPQVKDEVMRECFDCIRQGLG
YPSMRNDPILIANCMNWHGHPLEEARQWVHQACMSPCPSTKHGFQPFRMA... (6 Replies)
I have the following script:
awk 'FNR==NR{s+=$3;next;} { print $1 , $2, 100*$3/s }'
and the following file:
>P39PT-1224 Freq 900
cccctacgacggcattggtaatggctcagctgctccggatcccgcaagccatcttggatatgagggttcgtcggcctcttcagccaagg-cccccagcagaacatccagctgatcg
>P39PT-784 Freq 2... (2 Replies)
I would like to extract all entries containing the following patterns: ccccta & ccccccccc from the following infile:
>P39PT-1224_Freq_900
cccctacgacggcattggtaatggctcccgcaagccatctctcttcagccaagg
>P39PT-784_Freq_2
cccctacgacggcattggtaatggcacccgcaagccatctctcttccccccccc
>P39PT-678_Freq_5... (4 Replies)
Hi,
I have a fasta file with multiple sequences. How can i get only unique sequences from the file.
For example
my_file.fasta
>seq1
TCTCAAAGAAAGCTGTGCTGCATACTGTACAAAACTTTGTCTGGAGAGATGGAGAATCTCATTGACTTTACAGGTGTGGACGGTCTTCAGAGATGGCTCAAGCTAACATTCCCTGACACACCTATAGGGAAAGAGCTAAC
>seq2... (3 Replies)
Discussion started by: Ibk
3 Replies
LEARN ABOUT DEBIAN
devel::refcount
Devel::Refcount(3pm) User Contributed Perl Documentation Devel::Refcount(3pm)NAME
"Devel::Refcount" - obtain the REFCNT value of a referent
SYNOPSIS
use Devel::Refcount qw( refcount );
my $anon = [];
print "Anon ARRAY $anon has " . refcount($anon) . " reference
";
my $otherref = $anon;
print "Anon ARRAY $anon now has " . refcount($anon) . " references
";
DESCRIPTION
This module provides a single function which obtains the reference count of the object being pointed to by the passed reference value.
FUNCTIONS
$count = refcount($ref)
Returns the reference count of the object being pointed to by $ref.
COMPARISON WITH SvREFCNT
This function differs from "Devel::Peek::SvREFCNT" in that SvREFCNT() gives the reference count of the SV object itself that it is passed,
whereas refcount() gives the count of the object being pointed to. This allows it to give the count of any referent (i.e. ARRAY, HASH,
CODE, GLOB and Regexp types) as well.
Consider the following example program:
use Devel::Peek qw( SvREFCNT );
use Devel::Refcount qw( refcount );
sub printcount
{
my $name = shift;
printf "%30s has SvREFCNT=%d, refcount=%d
",
$name, SvREFCNT($_[0]), refcount($_[0]);
}
my $var = [];
printcount 'Initially, $var', $var;
my $othervar = $var;
printcount 'Before CODE ref, $var', $var;
printcount '$othervar', $othervar;
my $code = sub { undef $var };
printcount 'After CODE ref, $var', $var;
printcount '$othervar', $othervar;
This produces the output
Initially, $var has SvREFCNT=1, refcount=1
Before CODE ref, $var has SvREFCNT=1, refcount=2
$othervar has SvREFCNT=1, refcount=2
After CODE ref, $var has SvREFCNT=2, refcount=2
$othervar has SvREFCNT=1, refcount=2
Here, we see that SvREFCNT() counts the number of references to the SV object passed in as the scalar value - the $var or $othervar
respectively, whereas refcount() counts the number of reference values that point to the referent object - the anonymous ARRAY in this
case.
Before the CODE reference is constructed, both $var and $othervar have SvREFCNT() of 1, as they exist only in the current lexical pad. The
anonymous ARRAY has a refcount() of 2, because both $var and $othervar store a reference to it.
After the CODE reference is constructed, the $var variable now has an SvREFCNT() of 2, because it also appears in the lexical pad for the
new anonymous CODE block.
PURE-PERL FALLBACK
An XS implementation of this function is provided, and is used by default. If the XS library cannot be loaded, a fallback implementation in
pure perl using the "B" module is used instead. This will behave identically, but is much slower.
Rate pp xs
pp 225985/s -- -66%
xs 669570/s 196% --
SEE ALSO
o Test::Refcount - assert reference counts on objects
AUTHOR
Paul Evans <leonerd@leonerd.org.uk>
perl v5.14.2 2011-11-15 Devel::Refcount(3pm)