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Top Forums Shell Programming and Scripting parse fasta file to tabular file Post 302584570 by yifangt on Friday 23rd of December 2011 04:55:17 PM
Old 12-23-2011
parse fasta file to tabular file

Hello,
A bioperl problem I thought could be done with awk: convert the fasta format (Note: the length of each row is not the same for each entry as they were combined from different files!) to tabular format.
Code:
input.fasta:

>YAL069W-1.334 Putative promoter sequence
CCACACCACACCCACACACCCACACACCACACCACACACC
ACACCACACCCACACACACACATCCTAACACTACCCTAAC
ACAGCCCTAATCTAACCCTGGCCAACCTGTCTCTCAACTT
>YAL068C-7235.2170 Putative promoter sequence
TACGAGAATAATTTCTCATCATCCAGCTTTAACACAAAAT
ACGTAAATGAAGTTTATATATAAATTTCCTTTTTATTGGA
>gi|31044174|gb|AY143560.1| Tintinnopsis fimbriata 18S ribosomal RNA gene, partial sequence
GAAACTGCGAATGGCTCATTAAAACAGTTATAGTTTATTTGGTAATCAAACTTACATGGATAACCGTGG
TAATTCTAGAGCTAATACATGCTGTTGTGCCCGACTCACGAAGGGCGGTATTTATTAGATATCAGCCAATA
AGCATCTGCTATTGTGGTGACTCATAGTAACTTAATCGGATCGCATGGGCTTGTCCCGCGACAAACCATT
>gi|31044185|gb|AY143571.1| Codonellopsis americana 18S ribosomal RNA gene, partial sequence
ATTACCCAATCCTGACTCAGGGAGGTAGTGACAAGAAATAATGGGTCGGGGTTCTGCCCCGGGACTGCA
GGGCACCACCAGGCGTGGAGCTTGCGGCTCAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACAT

I want to convert it to the tabular format as:
Code:
output.tab:

>YAL069W-1.334 Putative promoter sequence CCACACCACACCCACACACCCACACA......CACAGCCCTAATCTAACCCTGGCCAACCTGTCTCTCAACTT
>YAL068C-7235.2170 Putative promoter sequence TACGAGAATAATTTCTCATCATCCAG......CATTTTCTTATGACGTAAATGAAGTTTATATATAAATTTCCTTTTTATTGGA
>gi|31044174|gb|AY143560.1| Tintinnopsis fimbriata 18S ribosomal RNA gene, partial sequence GAAACTGCGAATGGCTCA......ATTGTGGTGACTCATAGTAACTTAATCGGATCGCATGGGCTTGTCCCGCGACAAACCATT
>gi|31044185|gb|AY143571.1| Codonellopsis americana 18S ribosomal RNA gene, partial sequence ATTACCCAATCCTGACTC......CCAGGCGTGGAGCTTGCGGCTCAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACAT

i.e. each row has two columns: the first one is the header for the sequence name and description, the second column is the DNA sequence. This is quite common in bioinformatics daily task.
I am aware bioperl is the right tool to do the job, but I am trying to level up my awk when I read the RS variable. Not sure how to handle this situation for the RS and the FS variables.
Thanks a lot!
 

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Bio::SeqFeature::Gene::GeneStructureI(3pm)		User Contributed Perl Documentation		Bio::SeqFeature::Gene::GeneStructureI(3pm)

NAME
Bio::SeqFeature::Gene::GeneStructureI - A feature representing an arbitrarily complex structure of a gene SYNOPSIS
#documentation needed DESCRIPTION
A feature representing a gene structure. FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHOR - Hilmar Lapp Email hlapp@gmx.net APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ transcripts Title : transcripts() Usage : @transcripts = $gene->transcripts(); Function: Get the transcript features/sites of this gene structure. See Bio::SeqFeature::Gene::TranscriptI for properties of the returned objects. Returns : An array of Bio::SeqFeature::Gene::TranscriptI implementing objects representing the promoter regions or sites. Args : promoters Title : promoters() Usage : @prom_sites = $gene->promoters(); Function: Get the promoter features/sites of this gene structure. Note that OO-modeling of regulatory elements is not stable yet. This means that this method might change or even disappear in a future release. Be aware of this if you use it. Returns : An array of Bio::SeqFeatureI implementing objects representing the promoter regions or sites. Args : exons Title : exons() Usage : @exons = $gene->exons(); @inital = $gene->exons('Initial'); Function: Get all exon features or all exons of specified type of this gene structure. Refer to the documentation of the class that produced this gene structure object for information about the possible types. See Bio::SeqFeature::Gene::ExonI for properties of the returned objects. Returns : An array of Bio::SeqFeature::Gene::ExonI implementing objects representing the exon regions. Args : An optional string specifying the type of the exon. introns Title : introns() Usage : @introns = $gene->introns(); Function: Get all introns of this gene structure. Returns : An array of Bio::SeqFeatureI implementing objects representing the introns. Args : poly_A_sites Title : poly_A_sites() Usage : @polyAsites = $gene->poly_A_sites(); Function: Get the poly-adenylation features/sites of this gene structure. Returns : An array of Bio::SeqFeatureI implementing objects representing the poly-adenylation regions or sites. Args : utrs Title : utrs() Usage : @utr_sites = $gene->utrs(); Function: Get the UTR features/sites of this gene structure. See Bio::SeqFeature::Gene::ExonI for properties of the returned objects. Returns : An array of Bio::SeqFeature::Gene::ExonI implementing objects representing the UTR regions or sites. Args : perl v5.14.2 2012-03-02 Bio::SeqFeature::Gene::GeneStructureI(3pm)
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