06-23-2010
Trimming sequences based on specific pattern
My files look like this
Quote:
>GHXCZCC01AJ8CJ
TTGATGTGCTTGGTGTGTATCATTTCTGGGAAGCCCTACGCCCCGGGGC
>GHXCZCC01APUO5
T-ATGTGCCGTTGGTGTGTATCAGCTGGATTTCTGGGACGCAGCCCTACCCGGGGCGA
>GHXCZCC01AQSRP
TTGATGTTA---AGCTGGATTTTCTGGGACGCCCCGGGGAGCCCTA
>GHXCZCC01AQSRP
TTGTTGCCAGCTAGCTGAGCCCTAGATTTTCTGGGGCCCCGGGG
>GHXCZCC01AQSRP
TTGATGTTGCCCAGCCCTATAGCTGGATTTTCTGGGACGCCCCGGGGTGC
And I need to cut the sequences at the last "A" found in the following 'pattern' -highlighted for easier identification, the pattern is the actual file is not highlighted.
The expected result should look like this
Quote:
>GHXCZCC01AJ8CJ
TTGATGTGCTTGGTGTGTATCATTTCTGGGAAGCCCTA
>GHXCZCC01APUO5
T-ATGTGCCGTTGGTGTGTATCAGCTGGATTTCTGGGACGCAGCCCTA
>GHXCZCC01AQSRP
TTGATGTTA---AGCTGGATTTTCTGGGACGCCCCGGGGAGCCCTA
>GHXCZCC01AQSRP
TTGTTGCCAGCTAGCTGAGCCCTA
>GHXCZCC01AQSRP
TTGATGTTGCCCAGCCCTA
Thus, all the sequences would end with
AGCCCTA but whatever is to the left of that particular pattern and the identifiers (>GHXCZCC01AJ8CJ) should be kept intact.
Thanks in advance
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ppmtopgm(1) General Commands Manual ppmtopgm(1)
NAME
ppmtopgm - convert a portable pixmap into a portable graymap
SYNOPSIS
ppmtopgm [ppmfile]
DESCRIPTION
Reads a portable pixmap as input. Produces a portable graymap as output. The output is a "black and white" rendering of the original
image, as in a black and white photograph. The quantization formula used is .299 r + .587 g + .114 b.
Note that although there is a pgmtoppm program, it is not necessary for simple conversions from pgm to ppm , because any ppm program can
read pgm (and pbm ) files automatically. pgmtoppm is for colorizing a pgm file. Also, see ppmtorgb3 for a different way of converting
color to gray. And ppmdist generates a grayscale image from a color image, but in a way that makes it easy to differentiate the original
colors, not necessarily a way that looks like a black and white photograph.
QUOTE
Cold-hearted orb that rules the night
Removes the colors from our sight
Red is gray, and yellow white
But we decide which is right
And which is a quantization error.
SEE ALSO
pgmtoppm(1),ppmtorgb3(1),rgb3toppm(1),ppmdist(1),ppm(5),pgm(5)
AUTHOR
Copyright (C) 1989 by Jef Poskanzer.
10 April 2000 ppmtopgm(1)