06-17-2010
pseudocoder
Would it be a way to do the same with bash? It will be easier for me to understand.
I was wondering if there is any way to calculate the frequency of each sequence? In other words, let assume that after 'trimming' the sequences there are several that are identical, would it be possible to determine the frequency and include it as part of the ID line? Something like this:
Quote:
> Seq A Freq 50
AGAGATAGATAGAGCTGAT
> Seq B Freq 25
AGAGATAGATAGAGCTGAT
> Seq C Freq 25
AGAGATAGATAGAGCTGAT
Thanks
Last edited by Xterra; 06-17-2010 at 05:00 AM..
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LEARN ABOUT DEBIAN
fastx_clipper
FASTX_CLIPPER(1) User Commands FASTX_CLIPPER(1)
NAME
fastx_clipper - FASTA/Q Clipper
DESCRIPTION
usage: fastx_clipper [-h] [-a ADAPTER] [-D] [-l N] [-n] [-d N] [-c] [-C] [-o] [-v] [-z] [-i INFILE] [-o OUTFILE] Part of FASTX Toolkit
0.0.13.2 by A. Gordon (gordon@cshl.edu)
[-h] = This helpful help screen.
[-a ADAPTER] = ADAPTER string. default is CCTTAAGG (dummy adapter). [-l N] = discard sequences shorter than N nucleotides.
default is 5. [-d N] = Keep the adapter and N bases after it.
(using '-d 0' is the same as not using '-d' at all. which is the default).
[-c] = Discard non-clipped sequences (i.e. - keep only sequences which contained the adapter).
[-C] = Discard clipped sequences (i.e. - keep only sequences which did not contained the adapter).
[-k] = Report Adapter-Only sequences.
[-n] = keep sequences with unknown (N) nucleotides. default is to discard such sequences.
[-v] = Verbose - report number of sequences.
If [-o] is specified,
report will be printed to STDOUT.
If [-o] is not specified (and output goes to STDOUT), report will be printed to STDERR.
[-z] = Compress output with GZIP.
[-D] = DEBUG output.
[-M N] = require minimum adapter alignment length of N.
If less than N nucleotides aligned with the adapter - don't clip it.
[-i INFILE] = FASTA/Q input file. default is STDIN.
[-o OUTFILE] = FASTA/Q output file. default is STDOUT.
SEE ALSO
The quality of this automatically generated manpage might be insufficient. It is suggested to visit
http://hannonlab.cshl.edu/fastx_toolkit/commandline.html
to get a better layout as well as an overview about connected FASTX tools.
fastx_clipper 0.0.13.2 May 2012 FASTX_CLIPPER(1)