Sponsored Content
Top Forums Shell Programming and Scripting Extracting DNA sequences from GenBank files using Perl Post 302328326 by otheus on Wednesday 24th of June 2009 04:54:08 AM
Old 06-24-2009
I'll try to help with some code (one of those days):
Code:
($root,$gene) = (@ARGV);
open(TXT,$root.".txt") || die "Cannot open $root.txt";
open(GB,  $root.".gb") || die "Cannot open $root.gb";

$found=0;
while (<TXT>) { 
    ($start,$stop) = m/^$gene \((\d+)..(\d+)\)/io || next;
    $found = 1; last;
}
die "Did not find gene $gene in $root.txt" unless $found;

$found = 0;
while (<DB>) { 
  chop; 
  @x=split;
  if ($x[0] >= $start) { 
      # start-logic here;
  }
}
while (<DB>) { 
  chop; 
  @x=split;
  if ($x[0] < $stop) { 
      # stop-logic here
  }
}
# print logic here

 

10 More Discussions You Might Find Interesting

1. Shell Programming and Scripting

Perl - extracting data from .csv files

PROJECT: Extracting data from an employee timesheet. The timesheets are done in excel (for user ease) and then converted to .csv files that look like this (see color code key below): ,,,,,,,,,,,,,,,,,,, 9/14/2003,<-- Week Ending,,,,,,,,,,,,,,,,,, Craig Brennan,,,,,,,,,,,,,,,,,,,... (3 Replies)
Discussion started by: kregh99
3 Replies

2. Shell Programming and Scripting

GenBank Perl help...

Hey guys, I'm doing some Perl scripting for genomic data out of GenBank files...I have to extract the name of the plant, the file name, the number of bases, and all of the genes including their starting and ending positions...for example, with this GenBank file, LOCUS NC_010093 ... (7 Replies)
Discussion started by: akreibich07
7 Replies

3. Shell Programming and Scripting

To retrieve gene name & function thorugh Genbank id (gi|9910297)

Hi , I have list of genbank id's and ref number in this format. gi|9910297|ref|NM_019974.1| I want to retrive the gene name and fuction for each genbank list. I have around 1300 gi numbers in my excel sheet. So anybody can help me to retrive the information from NCBI through perl script... (0 Replies)
Discussion started by: shibujohn82
0 Replies

4. UNIX for Advanced & Expert Users

Extracting files with multiple links-perl

i want to write a perl script that gets/displays all those files having multiple links (in current directory) (4 Replies)
Discussion started by: guptesanket
4 Replies

5. Shell Programming and Scripting

Extracting column value from perl

Hello Kindly help me to find out the first column from first line of a flat file in perl I/P 9869912|20110830|00000000000013009|130|09|10/15/2010 12:36:22|W860944|N|00 9869912|20110830|00000000000013013|130|13|10/15/2010 12:36:22|W860944|N|00... (5 Replies)
Discussion started by: Pratik4891
5 Replies

6. Shell Programming and Scripting

Tricky task with DNA sequences.

I am trying to reverse and complement my DNA sequences. The file format is FASTA, something like this: Now, to reverse the sequence, I should start reading from right to left. At the same should be complemented. Thus, "A" should be read as "T"; "C" should be read as "G"; "T" should be converted... (8 Replies)
Discussion started by: Xterra
8 Replies

7. Shell Programming and Scripting

Randomly selecting sequences and generating specific output files

I have two files containing hundreds of different sequences with the same Identifiers (ID-001, ID-002, etc.,), something like this: Infile1: ID-001 ATGGGAGCGGGGGCGTCTGCCTTGAGGGGAGAGAAGCTAGATACA ID-002 ATGGGAGCGGGGGCGTCTGTTTTGAGGGGAGAGAAGCTAGATACA ID-003... (18 Replies)
Discussion started by: Xterra
18 Replies

8. Shell Programming and Scripting

Shell script for changing the accession number of DNA sequences in a FASTA file

Hi, I am having a file of dna sequences in fasta format which look like this: >admin_1_45 atatagcaga >admin_1_46 atatagcagaatatatat with many such thousands of sequences in a single file. I want to the replace the accession Id "admin_1_45" similarly in following sequences to... (5 Replies)
Discussion started by: margarita
5 Replies

9. Shell Programming and Scripting

Extraction of sequences from files

hey!!! I have 2 files file1 is as ids.txt and is >gi|546473186|gb|AWWX01630222.1| >gi|546473233|gb|AWWX01630175.1| >gi|546473323|gb|AWWX01630097.1| >gi|546474044|gb|AWWX01629456.1| >gi|546474165|gb|AWWX01629352.1| file2 is sequences.fasta and is like >gi|546473233|gb|AWWX01630175.1|... (9 Replies)
Discussion started by: harpreetmanku04
9 Replies

10. Shell Programming and Scripting

Convert a DNA sequence into Amino Acid

I am trying to write a bash script that would be able to read DNA sequences (each line in the file is a sequence) from a file, where sequences are separated by an empty line. I am then to find the amino acid that these DNA sequences encode per codon (each group of three literals.) For example, if I... (3 Replies)
Discussion started by: faizlo
3 Replies
Bio::HandlerBaseI(3pm)					User Contributed Perl Documentation				    Bio::HandlerBaseI(3pm)

NAME
Bio::HandlerBaseI - Interface class for handler methods which interact with any event-driven parsers (drivers). SYNOPSIS
# MyHandler is a Bio::HandlerBaseI-derived class for dealing with GenBank # sequence data, derived from a GenBank event-driven parser # inside a parser (driver) constructor $self->seqhandler($handler || MyHandler->new(-format => 'genbank')); # in the driver parsing method ( such as next_seq() ) ... $handler = $self->seqhandler(); # roll data up into hashref chunks, pass off into Handler for processing... $hobj->data_handler($data); # or retrieve Handler methods and pass data directly to Handler methods my $hmeth = $hobj->handler_methods; if ($hmeth->{ $data->{NAME} }) { my $mth = $hmeth->{ $data->{NAME} }; # code ref $hobj->$mth($data); } DESCRIPTION
This interface describes simple class methods used for processing data from an event-based parser (a driver). This is similar in theme to an XML SAX-based driver but differs in that one can optionally pass related data semi-intelligently as chunks (defined in a hash reference) vs. passing as single data elements in a stream. For instance, any reference-related and species-related data as well as individual sequence features could be passed as chunks of data to be processed in part or as a whole (from Data::Dumper output): Annotation Data (References): $VAR1 = { 'NAME' => 'REFERENCE', 'DATA' => '1 (bases 1 to 10001)' 'AUTHORS' => 'International Human Genome Sequencing Consortium.' 'TITLE' => 'The DNA sequence of Homo sapiens' 'JOURNAL' => 'Unpublished(2003)' }; Sequence features (source seqfeature): $VAR1 = { 'mol_type' => 'genomic DNA', 'LOCATION' => '<1..>10001', 'NAME' => 'FEATURES', 'FEATURE_KEY' => 'source', 'note' => 'Accession AL451081 sequenced by The Sanger Centre', 'db_xref' => 'taxon:9606', 'clone' => 'RP11-302I18', 'organism' => 'Homo sapiens' }; These would be 'handled' accordingly by methods specified in a HandlerI-based class. The data in a chunk is intentionally left vague here since this may vary from implementation to implementation and can be somewhat open to interpretation. A data chunk in a sequence record, for instance, will be different than a data chunk in a BLAST report. This also allows one the flexibility to pass data as more XML-like small bits, as huge chunks, or even as indexed locations in a file (such as when using a "pull" parser, like a Bio::PullParserI). For an sequence-based implementation see Bio::SeqIO::RichSeq::GenericRichSeqHandler, which handles any GenBank, UniProt, and EMBL data from their respective driver modules (Bio::SeqIO::gbdriver, Bio::SeqIO::swissdriver, and Bio::SeqIO::embldriver). FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHOR - Chris Fields Email cjfields at bioperl dot org APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ data_handler Title : data_handler Usage : $handler->data_handler($data) Function: Centralized method which accepts all data chunks, then distributes to the appropriate methods for processing based on the chunk name from within the HandlerBaseI object. One can also use Returns : None Args : an hash ref containing a data chunk. handler_methods Title : handler_methods Usage : $handler->handler_methods('GenBank') %handlers = $handler->handler_methods(); Function: Retrieve the handler methods used for the current format() in the handler. This assumes the handler methods are already described in the HandlerI-implementing class. Returns : a hash reference with the data type handled and the code ref associated with it. Args : [optional] String representing the sequence format. If set here this will also set sequence_format() Throws : On unimplemented sequence format in %HANDLERS format Title : format Usage : $handler->format('GenBank') $handler->format('BLAST') Function: Get/Set the format for the report/record being parsed. This can be used to set handlers in classes which are capable of processing similar data chunks from multiple driver modules. Returns : String with the sequence format Args : [optional] String with the sequence format Note : The format may be used to set the handlers (as in the current GenericRichSeqHandler implementation) get_params Title : get_params Usage : $handler->get_params('-species') Function: Convenience method used to retrieve the specified parameters from the internal parameter cache Returns : Hash ref containing parameters requested and data as key-value pairs. Note that some parameter values may be objects, arrays, etc. Args : List (array) representing the parameters requested set_params Title : set_params Usage : $handler->set_params({ '-species' => $species, '-accession_number' => $acc }); Function: Convenience method used to set specific parameters Returns : None Args : Hash ref containing the data to be passed as key-value pairs reset_parameters Title : reset_parameters Usage : $handler->reset_parameters() Function: Resets the internal cache of data (normally object parameters for a builder or factory) Returns : None Args : None perl v5.14.2 2012-03-02 Bio::HandlerBaseI(3pm)
All times are GMT -4. The time now is 03:31 PM.
Unix & Linux Forums Content Copyright 1993-2022. All Rights Reserved.
Privacy Policy