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Full Discussion: fasta format?
Top Forums UNIX for Dummies Questions & Answers fasta format? Post 302281007 by lost on Wednesday 28th of January 2009 03:35:21 AM
Old 01-28-2009
fasta format?

Hi,
I'm in need of creating a file in the fasta format:
Code:
>1A6A.A      
HVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITN
VPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCR
VEHWGLDEPLLKHWEF
>1A6A.B    
PRFLEYSTSECHFFNGTERVRYLDRYFHNQEENVRFDSDVGEFRAVTELGRPDAEYWNSQKDLLEQKRGRVDNYCRHNYG

Thinking it could be possible using awk... Just don't have it right at all below since it's only printing the first 60 positions in the line and not making the full line into several lines of 60 positions.
Code:
cat filename | awk '{print substr($1,1,60)}'

I also need to add a '>' to the first line with the lineID
Right now my file is in this format:
Code:
DRB1_010101
MVCLKLPGGSCMTALTVTLMVLSSPLALAGDTRPRFLWQLKFECHFFNGTERVRLLERCIYNQEESVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS

Help! and thanks!
 

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Bio::AlignIO::fasta(3pm)				User Contributed Perl Documentation				  Bio::AlignIO::fasta(3pm)

NAME
Bio::AlignIO::fasta - fasta MSA Sequence input/output stream SYNOPSIS
Do not use this module directly. Use it via the Bio::AlignIO class. DESCRIPTION
This object can transform Bio::SimpleAlign objects to and from fasta flat file databases. This is for the fasta alignment format, not for the FastA sequence analysis program. To process the alignments from FastA (FastX, FastN, FastP, tFastA, etc) use the Bio::SearchIO module. FEEDBACK
Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHORS
Peter Schattner APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ next_aln Title : next_aln Usage : $aln = $stream->next_aln Function: returns the next alignment in the stream. Returns : Bio::Align::AlignI object - returns 0 on end of file or on error Args : -width => optional argument to specify the width sequence will be written (60 chars by default) See Bio::Align::AlignI write_aln Title : write_aln Usage : $stream->write_aln(@aln) Function: writes the $aln object into the stream in fasta format Returns : 1 for success and 0 for error Args : L<Bio::Align::AlignI> object See Bio::Align::AlignI _get_len Title : _get_len Usage : Function: determine number of alphabetic chars Returns : integer Args : sequence string width Title : width Usage : $obj->width($newwidth) $width = $obj->width; Function: Get/set width of alignment Returns : integer value of width Args : on set, new value (a scalar or undef, optional) perl v5.14.2 2012-03-02 Bio::AlignIO::fasta(3pm)
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