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Does the above (reading the input file text_file) not work for you?

The value is stored in $my_var at the end of these few lines and you can use it as you please.

What is the eventual purpose? ...

It depends what you really want to do.

Do you want to convert this to an output file or string as 'ABC', 'BCD', 'CDF', 'DEF', 'EFG'

If so, you could:-while read line
do
my_var="$my_var,...