psequin(1) [debian man page]
PSEQUIN(1) NCBI Tools User's Manual PSEQUIN(1) NAME
Psequin - submit sequences to Genbank, EMBL, and DDBJ SYNOPSIS
Psequin [-b] [-bse] [-e] [-f filename] [-gc] [-h] [-oldaln] [-oldasn] [-oldgph] [-oldseq] [-oldsource] [-s] [-w] [-x] DESCRIPTION
Psequin is a program designed to aid in the submission of sequences to the GenBank, EMBL, and DDBJ sequence databases. It was written at the National Center for Biotechnology Information, part of the National Library of Medicine at the National Institutes of Health. Psequin can assemble the essential elements of a GenBank record from simple FASTA-format text files. For example, the program obtains the proper genetic code from an organism name, and automatically determines coding region intervals by back-translation from the protein sequence. An on-line help window scrolls to the appropriate place as the user moves between and within data entry forms, giving relevant details on what information is expected. Psequin also contains a number of built-in validation functions for quality assurance. Features such as splice sites and coding region translations are checked for accuracy or internal consistency. Double-clicking on an error message launches an appropriate editor by which the user can correct any problems. Psequin provides live, clickable views of the data in a variety of formats, including a report form, GenBank flatfile, EMBL flatfile, and a graphical view. Double clicking on an item in any of these formats launches an editor for that item. The editor is capable of maintaining correct feature table positions as the underlying sequence is edited. It can display features on the sequence during editing, and allows feature intervals to be adjusted by direct manipulation. OPTIONS
-b Bioseq-set mode -bse binseqentry mode -e Entrez mode -f filename read from filename -gc genome center mode -h turn off automatic help -oldaln use old alignment reader -oldasn leave as old ASN.1 -oldgph use old graphic view -oldseq use old sequence view -oldsource use old flat-file source format -s subtool mode -w workbench mode -x read from standard input AUTHOR
The National Center for Biotechnology Information. SEE ALSO
asnmacro(1), sbtedit(1), tbl2asn(1), /usr/share/doc/ncbi-tools-x11/sequin.htm, <http://www.ncbi.nlm.nih.gov/Sequin/>. NCBI
2011-09-02 PSEQUIN(1)
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ASN2ALL(1) NCBI Tools User's Manual ASN2ALL(1) NAME
asn2all - generate reports from ASN.1 biological data SYNOPSIS
asn2all [-] [-A acc] [-F filename] [-G] [-J n] [-K n] [-M] [-T] [-X] [-a type] [-b] [-c] [-d path] [-f format] [-h] [-i filename] [-k] [-l] [-n policy] [-o filename] [-p path] [-r] [-v filename] [-x ext] DESCRIPTION
asn2all is primarily intended for generating reports from the binary ASN.1 Bioseq-set release files downloaded from the NCBI ftp site (ncbi-asn1 directory). It can produce GenBank and GenPept flatfiles, FASTA sequence files, INSDSet structured XML, TinySeq XML, and Sequin-style 5-column feature tables. The release files (which have the extension .aso.gz) should be uncompressed with gunzip(1), resulting in files with the extension .aso. For example, gbpri1.aso is the first file in the primate division, and the command gunzip gbpri1.aso.gz will result in gbpri1.aso being created. The original gbpri1.aso.gz file is removed after successful decompression. In asn2all, the name of the file to be processed is specified by the -i command line argument. Use -a t to indicate that it is a release file and -b to indicate that it is binary ASN.1. A text ASN.1 file obtained from Entrez can be processed by using -a a instead of -a t -b. Nucleotide and protein records can be processed simultaneously. Use the -o argument to indicate the nucleotide output file, and the -v argument for the protein output file. The -f argument determines the format to be generated, and is documented in more detail (along with other options) in the following sec- tion. OPTIONS
A summary of options is included below. - Print usage message -A accession Accession to fetch; may take the form accession,complexity,flags where complexity should normally be 0 and a flags value of -1 enables fetching of external features -F filename Accession Filter file -G Relaxed Genome Mapping -J n Seq-loc from -K n Seq-loc to -M Seq-loc Minus strand -T Use Threads -X EXtended qualifier output -a type Input ASN.1 type: a Automatic (default) c Catenated z Any e Seq-entry b Bioseq s Bioseq-set m Seq-submit t batch processing (suitable for official releases; autodetects specific type) -b Bioseq-set is Binary -c Bioseq-set is Compressed -d path Path to indexed binary ASN.1 Data -f format Output Format: g GenBank/GenPept (default) m GenBank Master Style f FASTA d CDS FASTA e Gene FASTA t Sequin-style 5-column feature table y TinySet XML (akin to FASTA) s INSDSet XML (akin to GenBank/GenPept) a structurally equivalent text ASN.1 x structurally equivalent XML c cache components -h Display extra Help message -i filename Input file name (standard input by default) -k Enable local fetching -l Lock components in advance -n policy Near FASTA policy: a All n Near only (default) f Far only -o filename Nucleotide Output file name -p path Path to files -r Enable Remote fetching -v filename Protein output file name -x ext File selection suffix when working with entire directories. (default is .aso) EXAMPLES
The command asn2all -i gbpri1.aso -a t -b -f g -o gbpri1.nuc -v gbpri1.prt will generate GenBank and GenPept reports from gbpri1.aso. AUTHOR
The National Center for Biotechnology Information. SEE ALSO
asn2asn(1), asn2ff(1), asn2fsa(1), asn2gb(1), asn2idx(1), asn2xml(1), asndhuff(1), gene2xml(1), gunzip(1). NCBI
2012-06-24 ASN2ALL(1)