Is it possible to rename fasta headers based on its position specified in another file?

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Is it possible to rename fasta headers based on its position specified in another file?

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11-13-2019

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Is it possible to rename fasta headers based on its position specified in another file?

I have 5 sequences in a fasta file namely gene1.fasta as follows,

Code:

gene1.fasta
>1256
ATGTAGC
>GEP
TAGAG
>GTY578
ATGCATA
>67_iga
ATGCTGA
>90_ld
ATGCTG

I need to rename the gene1.fasta file based on the sequence position specified in list.txt as follows,

Code:

list.txt
position1=org5
position2=amylase
position3=org8
position4=lipase
position5=org_1

The expected outcome should be like this,

Code:

>org5
ATGTAGC
>amylase
TAGAG
>org8
ATGCATA
>lipase
ATGCTGA
>org_1
ATGCTG

Thanks in advance.

Last edited by dineshkumarsrk; 11-13-2019 at 03:07 AM..

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11-13-2019

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Any attempts / ideas / thoughts from your side? Applying what you learned in here?

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Dear Rudic,
Below script can rename the characters in gene1.fasta specified in list.txt.

Code:

awk 'FNR==NR{REP[$1]=$2; next} {for (r in REP) gsub(r, REP[r])}1' FS="=" list.txt gene1.fasta

However, it is not based on the position. Its purely based on the matching strings between the two files. But, here my problem is different, I tried workout like this

Code:

++i

, but my list.txt is not having common strings, so I can not rename sequentially. That is why I seek your help.

Last edited by dineshkumarsrk; 11-13-2019 at 04:12 AM..

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For the easy case that your replacements are in lines in increasing order, try

Code:

awk 'FNR==NR {REP[NR] = $2; next} /^>/ {$0 = ">" REP[++CNT]}1' FS="=" list.txt gene1.fasta
>org5
ATGTAGC
>amylase
TAGAG
>org8
ATGCATA
>lipase
ATGCTGA
>org_1
 ATGCTG

EDIT: in case it's not (here: position3 doesn't exist), try

Code:

awk 'FNR==NR {REP[$1] = $2; next} /^>/ && (TMP = "position" ++CNT) in REP {$0 = ">" REP[TMP]}1' FS="=" list.txt gene1.fasta
>org5
ATGTAGC
>amylase
TAGAG
>GTY578
ATGCATA
>lipase
ATGCTGA
>org_1
ATGCTG

Last edited by RudiC; 11-13-2019 at 05:10 AM..

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@RudiC,
Both serve my purpose perfectly.

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Also try:

Code:

awk -F= '/^>/{if(getline<f>0) $0=">" $2}1' f=list.txt gene1.fasta

or without the check:

Code:

awk -F= '/^>/{getline<f; $0=">" $2}1' f=list.txt gene1.fasta

Last edited by Scrutinizer; 11-14-2019 at 05:31 PM..

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