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Enviroment file is not a apache configuration file, try removing that line entirely.
Every time you change a systemd unit, you need to reload the daemon

systemctl daemon-reload


Then try to...

You have an error here i believe :

....
EnvironmentFile=/usr/local/apache-2.4.33/bin/httpd
.....


What i guess is going on, is that you are passing $OPTIONS as content of binary httpd file...