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bio::primerdesigner::tables(3pm) [debian man page]

Bio::PrimerDesigner::Tables(3pm)			User Contributed Perl Documentation			  Bio::PrimerDesigner::Tables(3pm)

Bio::PrimerDesigner::Table -- Draw HTML tables for PCR primer display DESCRIPTION
Draws simple HTML tables to display Bio::PrimerDesigner PCR primer design and e-PCR results for web applications. METHODS
info_table Prints a two-column table for generic, key-value style annotations. Expects to be passed the name of the gene/feature/etc. and a hash of attributes. If there is an 'image' key, the value is assumed to be an image URL, which is printed in a double-wide cell at the bottom of the table. my $gene = 'Abc-1'; my %gene_info = ( Chromosome => I, Start => 100450, Stop => 102893, Strand => '+' ); my $page = Bio::PrimerDesigner::Tables->new; $page->info_table( $gene, %gene_info ); PCR_header Returns a generic header for the PCR primer table. Does not expect any argumments. PCR_set Returns the top row for the PCR primer table. Expects the primer set number as its only argument. PCR_row Returns table rows with PCR primer info. Should be called once for each primer pair. Expects to be passed a hash containing the Bio::PrimerDesigner::Result object and the primer set number and an (optional) label. my $pcr_row = PCR_row( primers => $result_obj, setnum => $set_number, label => $label ); ePCR_row Returns table rows summarizing e-PCR results. Expects to be passed an Bio::PrimerDesigner::Result e-PCR results object and an optional e-PCR label. render Renders the image URL. Expects to be passed a hash of the map start and stop, and other features to be mapped (i.e. gene,forward_primer,reverse_primer, label,start and stop of each feature, and gene strand). my $image = $page->render( start => $startleft, stop => $startright, feat => $features ); PCR_map Returns a 6 column wide table cell with the <IMG ...> info. Will display the image of mapped primers in the browser when passed the image URL. AUTHOR
Copyright (C) 2003-2009 Sheldon McKay <>. LICENSE
This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; version 3 or any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA. SEE ALSO
Bio::PrimerDesigner::primer3, Bio::PrimerDesigner::epcr. perl v5.10.0 2009-08-04 Bio::PrimerDesigner::Tables(3pm)

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Bio::SeqFeature::Primer(3pm)				User Contributed Perl Documentation			      Bio::SeqFeature::Primer(3pm)

Bio::SeqFeature::Primer - Primer Generic SeqFeature SYNOPSIS
# set up a single primer that can be used in a PCR reaction use Bio::SeqFeature::Primer; # initiate a primer with raw sequence my $primer=Bio::SeqFeature::Primer->new(-seq=>'CTTTTCATTCTGACTGCAACG'); # get the primery tag for the primer # should return Primer my $tag=$primer->primary_tag; # get or set the location that the primer binds to the target at $primer->location(500); my $location=$primer->location(500); # get or set the 5' end of the primer homology, as the primer doesn't # have to be the same as the target sequence $primer->start(2); my $start=$primer->start; # get or set the 3' end of the primer homology $primer->end(19); my $end = $primer->end; # get or set the strand of the primer. Strand should be 1, 0, or -1 $primer->strand(-1); my $strand=$primer->strand; # get or set the id of the primer $primer->display_id('test_id'); my $id=$primer->display_id; # get the tm of the primer. This is calculated for you by the software. # however, see the docs. my $tm = $primer->Tm; print "These are the details of the primer: Tag: $tag Location $location Start: $start "; print " End: $end Strand: $strand ID: $id Tm: $tm "; DESCRIPTION
Handle primer sequences. This will allow you to generate a primer object required for a Bio::Seq::PrimedSeq object. This module is designed to integrate with Bio::Tools::Primer3 and Bio::Seq::PrimedSeq. In addition, you can calculate the melting temperature of the primer. This module is supposed to implement location and range, presumably through, but does not do so yet. However, it does allow you to set primers, and use those objects as the basis for Bio::Seq::PrimedSeq objects. See also the POD for Bio::Seq::PrimedSeq and Bio::Tools::Nucleotide::Analysis::Primer3 FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. - General discussion - About the mailing lists Support Please direct usage questions or support issues to the mailing list: rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: AUTHOR
Rob Edwards, The original concept and much of the code was written by Chad Matsalla, APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ new() Title : new() Usage : $primer = Bio::SeqFeature::Primer(-seq=>sequence_object); Function: Instantiate a new object Returns : A SeqPrimer object Args : You must pass either a sequence object (preferable) or a sequence. seq() Title : seq() Usage : $seq = $primer->seq(); Function: Return the sequence associated with this Primer. Returns : A Bio::Seq object Args : None. source_tag() Title : source_tag() Usage : $tag = $feature->source_tag(); Function: Returns the source of this tag. Returns : A string. Args : If an argument is provided, the source of this SeqFeature is set to that argument. location() Title : location() Usage : $tag = $primer->location(); Function: Gets or sets the location of the primer on the sequence Returns : If the location is set, returns that, if not returns 0. Note: At the moment I am using the primer3 notation of location (although you can set whatever you want). In this form, both primers are given from their 5' ends and a length. In this case, the left primer is given from the leftmost end, but the right primer is given from the rightmost end. You can use start() and end() to get the leftmost and rightmost base of each sequence. Args : If supplied will set a location start() Title : start() Usage : $start_position = $primer->start($new_position); Function: Return the start position of this Primer. This is the leftmost base, regardless of whether it is a left or right primer. Returns : The start position of this primer or 0 if not set. Args : If supplied will set a start position. end() Title : end() Usage : $end_position = $primer->end($new_position); Function: Return the end position of this primer. This is the rightmost base, regardless of whether it is a left or right primer. Returns : The end position of this primer. Args : If supplied will set an end position. strand() Title : strand() Usage : $strand=$primer->strand() Function: Get or set the strand. Returns : The strand that the primer binds to. Args : If an argument is supplied will set the strand, otherwise will return it. Should be 1, 0 (not set), or -1 display_id() Title : display_id() Usage : $id = $primer->display_id($new_id) Function: Returns the display ID for this Primer feature Returns : A scalar. Args : If an argument is provided, the display_id of this primer is set to that value. Tm() Title : Tm() Usage : $tm = $primer->Tm(-salt=>'0.05', -oligo=>'0.0000001') Function: Calculates and returns the Tm (melting temperature) of the primer Returns : A scalar containing the Tm. Args : -salt : set the Na+ concentration on which to base the calculation (default=0.05 molar). : -oligo : set the oligo concentration on which to base the calculation (default=0.00000025 molar). Notes : Calculation of Tm as per Allawi et. al Biochemistry 1997 36:10581-10594. Also see documentation at as they use this formula and have a couple nice help pages. These Tm values will be about are about 0.5-3 degrees off from those of the idtdna web tool. I don't know why. This was suggested by Barry Moore (thanks!). See the discussion on the bioperl-l with the subject "Bio::SeqFeature::Primer Calculating the PrimerTM" Tm_estimate Title : Tm_estimate Usage : $tm = $primer->Tm_estimate(-salt=>'0.05') Function: Calculates and returns the Tm (melting temperature) of the primer Returns : A scalar containing the Tm. Args : -salt set the Na+ concentration on which to base the calculation. Notes : This is an estimate of the Tm that is kept in for comparative reasons. You should probably use Tm instead! This Tm calculations are taken from the Primer3 docs: They are based on Bolton and McCarthy, PNAS 84:1390(1962) as presented in Sambrook, Fritsch and Maniatis, Molecular Cloning, p 11.46 (1989, CSHL Press). Tm = 81.5 + 16.6(log10([Na+])) + .41*(%GC) - 600/length where [Na+] is the molar sodium concentration, %GC is the %G+C of the sequence, and length is the length of the sequence. However.... I can never get this calculation to give me the same result as primer3 does. Don't ask why, I never figured it out. But I did want to include a Tm calculation here because I use these modules for other things besides reading primer3 output. The primer3 calculation is saved as 'PRIMER_LEFT_TM' or 'PRIMER_RIGHT_TM' and this calculation is saved as $primer->Tm so you can get both and average them! perl v5.14.2 2012-03-02 Bio::SeqFeature::Primer(3pm)
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