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hmmfetch(1)							   HMMER Manual 						       hmmfetch(1)

NAME
hmmfetch - retrieve profile HMM(s) from a file SYNOPSIS
hmmfetch [options] <hmmfile> <key> (retrieves HMM named <key>) hmmfetch -f [options] <hmmfile> <keyfile> (retrieves all HMMs listed in <keyfile>) hmmfetch --index [options] <hmmfile> (indexes <hmmfile> for fetching) DESCRIPTION
Quickly retrieves one or more profile HMMs from an <hmmfile> (a large Pfam database, for example). The <hmmfile> must be indexed first, using hmmfetch --index. The index is a binary file named <hmmfile>.ssi. The default mode is to retrieve a single profile by name or accession, called the <key>. For example: % hmmfetch Pfam-A.hmm Caudal_act % hmmfetch Pfam-A.hmm PF00045 With the -f option, a <keyfile> containing a list of one or more keys is read instead. The first whitespace-delimited field on each non- blank non-comment line of the <keyfile> is used as a <key>, and any remaining data on the line is ignored; this allows a variety of white- space delimited datafiles to be used as <keyfile>s. The <keyfile> argument can be a '-' (dash) character, in which case the keyfile is read from standard input; this allows unix ninjas to construct sophisticated invocations using pipes. By default, the HMM is printed to standard output in HMMER3 format. OPTIONS
-h Help; print a brief reminder of command line usage and all available options. -f The second commandline argument is a <keyfile> instead of a single <key>. The first field on each line of the <keyfile> is used as a retrieval <key> (an HMM name or accession). Blank lines and comment lines (that start with a # character) are ignored. -o <f> Output HMM(s) to file <f> instead of to standard output. -O Output HMM(s) to individual file(s) named <key> instead of standard output. With the -f option, this can result in many files being created. --index Instead of retrieving one or more profiles from <hmmfile>, index the <hmmfile> for future retrievals. This creates a <hmmfile>.ssi binary index file. SEE ALSO
See hmmer(1) for a master man page with a list of all the individual man pages for programs in the HMMER package. For complete documentation, see the user guide that came with your HMMER distribution (Userguide.pdf); or see the HMMER web page (@HMMER_URL@). COPYRIGHT
@HMMER_COPYRIGHT@ @HMMER_LICENSE@ For additional information on copyright and licensing, see the file called COPYRIGHT in your HMMER source distribution, or see the HMMER web page (@HMMER_URL@). AUTHOR
Eddy/Rivas Laboratory Janelia Farm Research Campus 19700 Helix Drive Ashburn VA 20147 USA http://eddylab.org HMMER
@HMMER_VERSION@ @HMMER_DATE@ hmmfetch(1)

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hmmalign(1)							   HMMER Manual 						       hmmalign(1)

NAME
hmmalign - align sequences to a profile HMM SYNOPSIS
hmmalign [options] <hmmfile> <seqfile> DESCRIPTION
Perform a multiple sequence alignment of all the sequences in seqfile, by aligning them individually to the profile HMM in hmmfile. The new alignment is output to stdout in Stockholm format. The sequences in seqfile are aligned in unihit local alignment mode. Therefore they should already be known to contain a single domain; they should not contain more than one domain. They may be fragments. The optimal alignment may assign some residues as nonhomologous (N and C states), in which case these residues are still included in the resulting alignment, but shoved to the outer edges. To trim these nonhomologous residues from the result, see the --trim option. OPTIONS
-h Help; print a brief reminder of command line usage and all available options. -o <f> Direct the output alignment to file <f>, rather than to stdout. --allcol Include columns in the output alignment for every match (consensus) state in the hmmfile, even if it means having all-gap columns. This is useful in analysis pipelines that need to be able to maintain a predetermined profile HMM architecture (with an unchanging number of consensus columns) through an hmmalign step. --mapali <f> Merge the existing alignment in file <f> into the result, where <f> is exactly the same alignment that was used to build the model in hmmfile. This is done using a map of alignment columns to consensus profile positions that is stored in the hmmfile. The multi- ple alignment in <f> will be exactly reproduced in its consensus columns (as defined by the profile), but the displayed alignment in insert columns may be altered, because insertions relative to a profile are considered by convention to be unaligned data. --trim Trim nonhomologous residues (assigned to N and C states in the optimal alignments) from the resulting multiple alignment output. --amino Specify that all sequences in seqfile are proteins. By default, alphabet type is autodetected from looking at the residue composi- tion. --dna Specify that all sequences in seqfile are DNAs. --rna Specify that all sequences in seqfile are RNAs. --informat <s> Declare that the input seqfile is in format <s>. Accepted sequence file formats include FASTA, EMBL, Genbank, DDBJ, Uniprot, Stock- holm, and SELEX. Default is to autodetect the format of the file. --outformat <s> Specify that the msafile is in format <s>. Currently the accepted multiple alignment sequence file formats only include Stockholm and SELEX. Default is to autodetect the format of the file. SEE ALSO
See hmmer(1) for a master man page with a list of all the individual man pages for programs in the HMMER package. For complete documentation, see the user guide that came with your HMMER distribution (Userguide.pdf); or see the HMMER web page (@HMMER_URL@). COPYRIGHT
@HMMER_COPYRIGHT@ @HMMER_LICENSE@ For additional information on copyright and licensing, see the file called COPYRIGHT in your HMMER source distribution, or see the HMMER web page (@HMMER_URL@). AUTHOR
Eddy/Rivas Laboratory Janelia Farm Research Campus 19700 Helix Drive Ashburn VA 20147 USA http://eddylab.org HMMER
@HMMER_VERSION@ @HMMER_DATE@ hmmalign(1)
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