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Full Discussion: Help with a bash loop script
Top Forums UNIX for Beginners Questions & Answers Help with a bash loop script Post 303037350 by dre on Tuesday 30th of July 2019 09:34:25 AM
Old 07-30-2019
Help with a bash loop script

Create a single bash script that does the following:
a. Print out the number of occurrences for each motif that is found in the bacterial genome and output to a file called motif_count.txt
b. Create a fasta file for each motif (so 3 in total) which contains all of the genes and their corresponding sequences that have that motif. Each file should be named after the motif (ie ATG.txt) and outputted to a new directory called motifs

motif file is a txt file with these motifs : ATG, GGGGG, ATTTT
the bacterial genome file is fasta file with the following lines
Code:
 >gene1
GAAACTCGGTGTTGGCTTACCGGTCATTCCGAGCGTCATTTGGTTTTCGCGTCGTGGCGAAATGTGGTTCTACTACTCGTGGTGTATGCACTATTTATCCGGAATGTTCAGAGCGAGTAGACAATGGGTGCTCCACAATTGTGGCGGTCCCTAAGGGACTCACATATAGTGAGACACGCGTGAAATTCTGCTCACCACGTCCGAATCCGACAAATCATCTACTTCGACGGTA
>gene2
CGGAGATAAAGGACCCATACTGTACGACATTGTATTGCTCACCATGGTCAATCTTTGCGAGTTGTTGCAGCTCGCAGCTTCGTTCTGTCAATATAGCTTAGATACTGAGAAGAAGTTGCAGAGAAAGTCGCA

Moderator's Comments:
Mod Comment edit by bakunin: please use CODE-tags to let data, code and terminal output stand out. Thank you.

Last edited by bakunin; 07-30-2019 at 10:48 AM..
 

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GLAM2FORMAT(1)							   glam2 Manual 						    GLAM2FORMAT(1)

NAME
glam2format - converts GLAM2 motifs to FASTA or MSF format SYNOPSIS
glam2format [options] my_format my_motif.glam2 Formats: fasta, msf. DESCRIPTION
glam2format reads in a motif found by glam2, and writes it in a standard alignment format (FASTA-with-gaps or MSF). This enables the alignment to be passed to third-party software, including graphical visualization tools such as Kalignvu, Boxshade, and WebLogo. On the other hand, not all the motif information is preserved: in particular, the key positions are lost. Only the top motif in glam2 output is converted. OPTIONS (DEFAULT SETTINGS) -o Output file (stdout). -c Make a compact alignment. By default, residues that are inserted between key positions are written as unaligned with each other. This best reflects glam2's intention, but it can make the alignment large and full of gaps. With -c, inserted residues are written as arbitrarily aligned with each other, just as they appear in the glam2 output. -f Sequence file to make a "global" alignment by adding flanking sequences from the original FASTA-format sequence file. The flanking sequences will be written as either unaligned with each other or arbitrarily aligned, depending on the -c option. The sequences should have unique names and their order should be unchanged. SEE ALSO
boxshade(1), glam2(1), glam2mask(1), glam2-purge(1), glam2scan(1) The full Hypertext documentation of GLAM2 is available online at http://bioinformatics.org.au/glam2/ or on this computer in /usr/share/doc/glam2/. REFERENCE
If you use GLAM2, please cite: MC Frith, NFW Saunders, B Kobe, TL Bailey (2008) Discovering sequence motifs with arbitrary insertions and deletions, PLoS Computational Biology (in press). AUTHORS
Martin Frith Author of GLAM2. Timothy Bailey Author of GLAM2. Charles Plessy <plessy@debian.org> Formatted this manpage in DocBook XML for the Debian distribution. COPYRIGHT
The source code and the documentation of GLAM2 are released in the public domain. GLAM2 1056 05/19/2008 GLAM2FORMAT(1)
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