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Top Forums UNIX for Dummies Questions & Answers Using grep and zgrep then display the next few lines Post 302448214 by khestoi on Wednesday 25th of August 2010 01:07:26 PM
Old 08-25-2010
Nope. The -A option is not available.
I tried the awk command and it works but it will only yeild one result.
 

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PERLPRIMER(1)						      General Commands Manual						     PERLPRIMER(1)

NAME
perlprimer - graphically specify amplicon of DNA or mRNA sequences and design primers SYNOPSIS
perlprimer DESCRIPTION
PerlPrimer calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. To adjust for the salt conditions of the PCR, PerlPrimer uses the empirical formula derived by von Ahsen, et al. (2001) and allows the user to specify the concentration of Mg2+, dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting temperature, giving rise to a maximum yeild of product when amplified. PerlPrimer is written in Perl and requires Perl/Tk. In addition, for QPCR functionality PerlPrimer requires the open-source Spidey executable from NCBI. The program is designed to be cross-platform com- patible and has been developed and tested on both Microsoft Windows and GNU/Linux-based operating systems. Users have also reported success using the program under Mac OS X. SEE ALSO
A very nice tutorial on http://perlprimer.sourceforge.net is also distributed as a debian package perlprimer-doc. AUTHOR
perlprimer was written by Owen Marshall <owenjm@users.sourceforge.net> This manual page was assembled by Steffen Moeller <steffen_moeller@gmx.de>, for the Debian project (but may be used by others). 6 January 2005 PERLPRIMER(1)
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