bp_make_mrna_protein(1p) [debian man page]
BP_MAKE_MRNA_PROTEIN(1p) User Contributed Perl Documentation BP_MAKE_MRNA_PROTEIN(1p) NAME
make_mrna_protein - Convert an input mRNA/cDNA sequence into protein DESCRIPTION
Convert an input mRNA/cDNA sequence into protein using translate() -f/--frame Specifies frame [0,1,2] One can also specify: -t/--terminator Stop Codon character (defaults to '*') -u/--unknown Unknown Protein character (defaults to 'X') -cds/--fullcds Expected Full CDS (with start and Stop codon) -throwOnError Throw error if no Full CDS (defaults to 0) -if/--format Input format (defaults to FASTA/Pearson) -of/--format Output format (defaults to FASTA/Pearson) -o/--output Output Filename (defaults to STDOUT) -i/--input Input Filename (defaults to STDIN) -ct/--codontable Codon table to use (defaults to '1') See Bio::PrimarySeq for more information on codon tables and the translate() method AUTHOR - Jason Stajich Email jason-at-bioperl-dot-org perl v5.14.2 2012-03-02 BP_MAKE_MRNA_PROTEIN(1p)
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Bio::Tools::CodonTable(3pm) User Contributed Perl Documentation Bio::Tools::CodonTable(3pm) NAME
Bio::Tools::CodonTable - Codon table object SYNOPSIS
# This is a read-only class for all known codon tables. The IDs are # the ones used by nucleotide sequence databases. All common IUPAC # ambiguity codes for DNA, RNA and amino acids are recognized. use Bio::Tools::CodonTable; # defaults to ID 1 "Standard" $myCodonTable = Bio::Tools::CodonTable->new(); $myCodonTable2 = Bio::Tools::CodonTable->new( -id => 3 ); # change codon table $myCodonTable->id(5); # examine codon table print join (' ', "The name of the codon table no.", $myCodonTable->id(4), "is:", $myCodonTable->name(), " "); # print possible codon tables $tables = Bio::Tools::CodonTable->tables; while ( ($id,$name) = each %{$tables} ) { print "$id = $name "; } # translate a codon $aa = $myCodonTable->translate('ACU'); $aa = $myCodonTable->translate('act'); $aa = $myCodonTable->translate('ytr'); # reverse translate an amino acid @codons = $myCodonTable->revtranslate('A'); @codons = $myCodonTable->revtranslate('Ser'); @codons = $myCodonTable->revtranslate('Glx'); @codons = $myCodonTable->revtranslate('cYS', 'rna'); # reverse translate an entire amino acid sequence into a IUPAC # nucleotide string my $seqobj = Bio::PrimarySeq->new(-seq => 'FHGERHEL'); my $iupac_str = $myCodonTable->reverse_translate_all($seqobj); # boolean tests print "Is a start " if $myCodonTable->is_start_codon('ATG'); print "Is a terminator " if $myCodonTable->is_ter_codon('tar'); print "Is a unknown " if $myCodonTable->is_unknown_codon('JTG'); DESCRIPTION
Codon tables are also called translation tables or genetic codes since that is what they represent. A bit more complete picture of the full complexity of codon usage in various taxonomic groups is presented at the NCBI Genetic Codes Home page. CodonTable is a BioPerl class that knows all current translation tables that are used by primary nucleotide sequence databases (GenBank, EMBL and DDBJ). It provides methods to output information about tables and relationships between codons and amino acids. This class and its methods recognized all common IUPAC ambiguity codes for DNA, RNA and animo acids. The translation method follows the conventions in EMBL and TREMBL databases. It is a nuisance to separate RNA and cDNA representations of nucleic acid transcripts. The CodonTable object accepts codons of both type as input and allows the user to set the mode for output when reverse translating. Its default for output is DNA. Note: This class deals primarily with individual codons and amino acids. However in the interest of speed you can translate longer sequence, too. The full complexity of protein translation is tackled by Bio::PrimarySeqI::translate. The amino acid codes are IUPAC recommendations for common amino acids: A Ala Alanine R Arg Arginine N Asn Asparagine D Asp Aspartic acid C Cys Cysteine Q Gln Glutamine E Glu Glutamic acid G Gly Glycine H His Histidine I Ile Isoleucine L Leu Leucine K Lys Lysine M Met Methionine F Phe Phenylalanine P Pro Proline O Pyl Pyrrolysine (22nd amino acid) U Sec Selenocysteine (21st amino acid) S Ser Serine T Thr Threonine W Trp Tryptophan Y Tyr Tyrosine V Val Valine B Asx Aspartic acid or Asparagine Z Glx Glutamine or Glutamic acid J Xle Isoleucine or Valine (mass spec ambiguity) X Xaa Any or unknown amino acid It is worth noting that, "Bacterial" codon table no. 11 produces an polypeptide that is, confusingly, identical to the standard one. The only differences are in available initiator codons. NCBI Genetic Codes home page: http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi?mode=c EBI Translation Table Viewer: http://www.ebi.ac.uk/cgi-bin/mutations/trtables.cgi Amended ASN.1 version with ids 16 and 21 is at: ftp://ftp.ebi.ac.uk/pub/databases/geneticcode/ Thanks to Matteo diTomasso for the original Perl implementation of these tables. FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHOR - Heikki Lehvaslaiho Email: heikki-at-bioperl-dot-org APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ id Title : id Usage : $obj->id(3); $id_integer = $obj->id(); Function: Sets or returns the id of the translation table. IDs are integers from 1 to 15, excluding 7 and 8 which have been removed as redundant. If an invalid ID is given the method returns 0, false. Example : Returns : value of id, a scalar, 0 if not a valid Args : newvalue (optional) name Title : name Usage : $obj->name() Function: returns the descriptive name of the translation table Example : Returns : A string Args : None tables Title : tables Usage : $obj->tables() or Bio::Tools::CodonTable->tables() Function: returns a hash reference where each key is a valid codon table id() number, and each value is the corresponding codon table name() string Example : Returns : A hashref Args : None translate Title : translate Usage : $obj->translate('YTR') Function: Returns a string of one letter amino acid codes from nucleotide sequence input. The imput can be of any length. Returns 'X' for unknown codons and codons that code for more than one amino acid. Returns an empty string if input is not three characters long. Exceptions for these are: - IUPAC amino acid code B for Aspartic Acid and Asparagine, is used. - IUPAC amino acid code Z for Glutamic Acid, Glutamine is used. - if the codon is two nucleotides long and if by adding an a third character 'N', it codes for a single amino acid (with exceptions above), return that, otherwise return empty string. Returns empty string for other input strings that are not three characters long. Example : Returns : a string of one letter ambiguous IUPAC amino acid codes Args : ambiguous IUPAC nucleotide string translate_strict Title : translate_strict Usage : $obj->translate_strict('ACT') Function: returns one letter amino acid code for a codon input Fast and simple translation. User is responsible to resolve ambiguous nucleotide codes before calling this method. Returns 'X' for unknown codons and an empty string for input strings that are not three characters long. It is not recommended to use this method in a production environment. Use method translate, instead. Example : Returns : A string Args : a codon = a three nucleotide character string revtranslate Title : revtranslate Usage : $obj->revtranslate('G') Function: returns codons for an amino acid Returns an empty string for unknown amino acid codes. Ambiguous IUPAC codes Asx,B, (Asp,D; Asn,N) and Glx,Z (Glu,E; Gln,Q) are resolved. Both single and three letter amino acid codes are accepted. '*' and 'Ter' are used for terminator. By default, the output codons are shown in DNA. If the output is needed in RNA (tr/t/u/), add a second argument 'RNA'. Example : $obj->revtranslate('Gly', 'RNA') Returns : An array of three lower case letter strings i.e. codons Args : amino acid, 'RNA' reverse_translate_all Title : reverse_translate_all Usage : my $iup_str = $cttable->reverse_translate_all($seq_object) my $iup_str = $cttable->reverse_translate_all($seq_object, $cutable, 15); Function: reverse translates a protein sequence into IUPAC nucleotide sequence. An 'X' in the protein sequence is converted to 'NNN' in the nucleotide sequence. Returns : a string Args : a Bio::PrimarySeqI compatible object (mandatory) a Bio::CodonUsage::Table object and a threshold if only codons with a relative frequency above the threshold are to be considered. reverse_translate_best Title : reverse_translate_best Usage : my $str = $cttable->reverse_translate_best($seq_object,$cutable); Function: Reverse translates a protein sequence into plain nucleotide sequence (GATC), uses the most common codon for each amino acid Returns : A string Args : A Bio::PrimarySeqI compatible object and a Bio::CodonUsage::Table object is_start_codon Title : is_start_codon Usage : $obj->is_start_codon('ATG') Function: returns true(1) for all codons that can be used as a translation start, false(0) for others. Example : $myCodonTable->is_start_codon('ATG') Returns : boolean Args : codon is_ter_codon Title : is_ter_codon Usage : $obj->is_ter_codon('GAA') Function: returns true(1) for all codons that can be used as a translation tarminator, false(0) for others. Example : $myCodonTable->is_ter_codon('ATG') Returns : boolean Args : codon is_unknown_codon Title : is_unknown_codon Usage : $obj->is_unknown_codon('GAJ') Function: returns false(0) for all codons that are valid, true(1) for others. Example : $myCodonTable->is_unknown_codon('NTG') Returns : boolean Args : codon unambiguous_codons Title : unambiguous_codons Usage : @codons = $self->unambiguous_codons('ACN') Returns : array of strings (one-letter unambiguous amino acid codes) Args : a codon = a three IUPAC nucleotide character string _unambiquous_codons deprecated, now an alias for unambiguous_codons add_table Title : add_table Usage : $newid = $ct->add_table($name, $table, $starts) Function: Add a custom Codon Table into the object. Know what you are doing, only the length of the argument strings is checked! Returns : the id of the new codon table Args : name, a string, optional (can be empty) table, a string of 64 characters startcodons, a string of 64 characters, defaults to standard perl v5.14.2 2012-03-02 Bio::Tools::CodonTable(3pm)