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T-COFFEE(1)						      General Commands Manual						       T-COFFEE(1)

NAME
t-coffee - multiple sequence alignment SYNOPSIS
t_coffee [options]file t-coffee aligns multiple DNA or protein sequences. OPTIONS
A summary of options is included below. For a complete description, see the documentation. -full_log S [0] -run_name S [0] -mem_mode S [0] mem -extend D [1] 1 -extend_mode S [0] very_fast_triplet -max_n_pair D [0] 10 -seq_name_for_quadruplet S [0] all -compact S [0] default -clean S [0] no -do_self FL [0] 0 -do_normalise D [0] 1000 -template_file S [0] -seq S [0] -in S [0] Mlalign_id_pair Mslow_pair -pdb S [0] -out_lib W_F [0] no -lib_only D [0] 0 -outseqweight W_F [0] no -seq_source S [0] ANY -cosmetic_penalty D [0] -50 -gapopen D [0] 0 -gapext D [0] 0 -fgapopen D [0] 0 -fgapext D [0] 0 -nomatch D [0] 0 -newtree W_F [0] default -usetree R_F [0] -tree_mode S [0] slow -quicktree FL [0] 0 -outfile W_F [0] default -maximise FL [1] 1 -output S [0] clustalw -infile R_F [0] -matrix S [0] blosum62mt -tg_mode D [0] 1 -profile_mode S [0] cw_profile_profile -profile_comparison S [0] full50 -dp_mode S [0] cfasta_pair_wise -ktuple D [0] 1 -ndiag D [0] 0 -diag_threshold D [0] 0 -diag_mode D [0] 0 -sim_matrix S [0] vasiliky -type S [0] -outorder S [0] aligned -seqnos S [0] off -case S [0] keep -cpu D [0] 0 -maxnseq D [0] 60 -maxlen D [0] -1 -weight S [0] default -seq_weight S [0] t_coffee -align FL [1] 1 -mocca FL [0] 0 -domain FL [0] 0 -start D [0] 0 -len D [0] 0 -scale D [0] 0 -mocca_interactive FL [0] 0 -evaluate_mode S [0] t_coffee_fast -get_type FL [0] 0 -clean_aln D [0] 0 -clean_threshold D [1] 1 -clean_iteration D [1] 1 -clean_evaluate_mode S [0] t_coffee_fast -profile S [0] -profile1 S [0] -profile2 S [0] -extend_matrix FL [0] 0 -prot_min_sim D [40] 40 -prot_max_sim D [60] 60 -prot_min_cov D [0] 0 -pdb_min_sim D [30] 30 -pdb_max_sim D [100] 100 -pdb_min_cov D [50] 50 -pdb_blast_server W_F [0] SIB -prot_blast_server W_F [0] SIB -pdb_db W_F [0] nrl3d -protein_db W_F [0] nr -method_log W_F [0] no -struc_to_use S [0] -cache W_F [0] use -align_pdb_param_file W_F [0] no -align_pdb_hasch_mode W_F [0] hasch_ca_trace_bubble -msa_mode S [0] tree -lalign_n_top D [0] 10 -iterate D [0] 0 -trim D [0] 0 -split D [0] 0 -trimfile S [0] default -split D [0] 0 -split_nseq_thres D [0] 0 -split_score_thres D [0] 0 -check_pdb_status D [0] 0 -seq_to_keep S [0] -dpa_master_aln S [0] -dpa_maxnseq D [0] 10 -dpa_min_score1 D [0] -dpa_min_score2 D [0] -dpa_keep_tmpfile FL [0] 0 -dpa_debug D [0] 0 -multi_thread S [0] -lib_list S [0] SEE ALSO
clustalw(1), /usr/share/doc/t-coffee/, http://igs-server.cnrs-mrs.fr/~cnotred/Projects_home_page/t_coffee_home AUTHOR
t-coffee was written by Cedric Notredame <cedric.notredame@europe.com> This manual page was written by Steffen Moeller <moeller@pzr.uni-rostock.de>, for the Debian project (but may be used by others). May 13, 2005 T-COFFEE(1)

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PROBALIGN(1)							   User Commands						      PROBALIGN(1)

NAME
probalign - Align sequences in MFAFILE(s) and print result to standard output DESCRIPTION
PROBALIGN Version 1.4 (Nov 2010) aligns multiple protein sequences and prints to the standard output. Written by Satish Chikkagoudar and Usman Roshan using code from PROBCONS version 1.1 (written by Chuong Do) and based upon probA (written by Ulrike Muckstein). PROBALIGN 1.4 comes with ABSOLUTELY NO WARRANTY. This is free software, and you are welcome to redistribute it under certain conditions. See the README file for details. Usage: probalign [OPTION]... [MFAFILE]... Description: Align sequences in MFAFILE(s) and print result to standard output -clustalw use CLUSTALW output format instead of MFA -v, --verbose report progress while aligning (default: off) -a, --alignment-order print sequences in alignment order rather than input order (default: off) -T, -temperature Sets the thermodynamic temperature parameter (default: 5 (for protein data mode), 1 ( for nucleotide data mode)). -score_matrix, --score_matrix Sets the type of scoring matrix used to calculate the posterior probabilities (default: gonnet_160, representing gonnet 160, refer README for details) -go, --gap-open This option can be used to specify the gap open parameter. The default for Gonnet 160 (protein) is 22 and nucleotide (simple matrix) is 4. -ge, --gap-extension This option can be used to specify the gap extension parameter. The default for Gonnet 160 (protein) is 1 and nucleotide (simple matrix) is 0.25. -nuc Specify this option to indicate that inputted sequences are nucleotide sequences -prot Specify this option to indicate that inputted sequences are protein sequences [DEFAULT] -showPP Outputs the posterior probabilities of alignment columns as a new sequence named Posterior Probabilities (The probability values are scaled to be between integers between 0 and 9). probalign 1.4 May 2011 PROBALIGN(1)
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