REPROF(1) User Commands REPROF(1)NAME
reprof - predict protein secondary structure and solvent accessibility
SYNOPSIS
reprof -i [query.blastPsiMat] [OPTIONS]
reprof -i [query.fasta] [OPTIONS]
reprof -i [query.blastPsiMat|query.fasta] --mutations [mutations.txt] [OPTIONS]
DESCRIPTION
Predict protein secondary structure and solvent accessibility.
Output Format
The output format is self-explanatory, i.e. the colums of the output are described in the output file itself.
OPTIONS -i, --input=FILE
Input BLAST PSSM matrix file (from Blast -Q option) or input (single) FASTA file.
-o, --out=FILE
Either an output file or a directory. If not provided or a directory, the suffix of the input filename (i.e. .fasta or .blastPsiMat) is
replaced to create an output filename.
--mutations=[all|FILE]
Either the keyword "all" to predict all possible mutations or a file containing mutations one per line such as "C12M" for C is mutated
to M on position 12:
C30Y
R31W
G48D
This mutation code is also attached to the output filename using "_". An additional file ending "_ORI" contains the prediction using
no evolutionary information even if a BLAST PSSM matrix was provided.
--modeldir=DIR
Directory where the model and feature files are stored. Default: /usr/share/reprof.
AUTHOR
Peter Hoenigschmid hoenigschmid@rostlab.org, Burkhard Rost
EXAMPLES
Prediction from BLAST PSSM matrix for best results:
reprof -i /usr/share/doc/reprof/examples/example.Q -o /tmp/example.Q.reprof
Prediction from FASTA file:
reprof -i /usr/share/doc/reprof/examples/example.fasta -o /tmp/example.fasta.reprof
Prediction from BLAST PSSM matrix file using the mutation mode:
reprof -i /usr/share/doc/reprof/examples/example.Q -o /tmp/mutations_example.Q.reprof --mutations /usr/share/doc/reprof/examples/mutations.txt
# Result files for the above call are going to be:
# /tmp/mutations_example.Q.{reprof,reprof_F172P,reprof_M1Q,reprof_N34Y,reprof_ORI} - see --mutations for a description of the extensions.
COPYRIGHT
This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.
You should have received a copy of the GNU General Public License along with this program. If not, see <http://www.gnu.org/licenses/>.
BUGS
https://rostlab.org/bugzilla3/enter_bug.cgi?product=reprof
SEE ALSO blast2(1)
http://rostlab.org/
1.0.1 2012-01-13 REPROF(1)
Check Out this Related Man Page
PROFTMB(1) User Commands PROFTMB(1)NAME
proftmb - per-residue prediction of bacterial transmembrane beta barrels
SYNOPSIS
proftmb [options]
DESCRIPTION
proftmb predicts bacterial transmembrane beta barrels from sequence using profile fed Hidden Markov Models (HMM).
Output format
Self-annotating. Letters of prediction: 'i' - inside cytoplasm, 'O' - outside cytoplasm, 'U' - up (on chain moving out of cytoplasm), 'D'
- down (on chain moving into cytoplasm).
References
Bigelow, H. and Rost, B. (2006). PROFtmb: a web server for predicting bacterial transmembrane beta barrel proteins. Nucleic Acids Res,
34(Web Server issue), W186-8.
To invoke the menu, type:
proftmb --menu
The options are:
-d, --directory-root <STRING>
root path where files (options -s,-r,-l,-a,-e,-t,-u,-z,-n) reside
-a, --reduction-state-decode <STRING>
state reduction for decoding
-b, --reduction-state-report <STRING>
state reduction for reporting
-m, --membrane-strand-states <STRING>
list of membrane strand states
-z, --z-curve-file
<STRING> file containing means and sd's at integral length values
-x, --z-calibration-curve <STRING>
file mapping coverage and accuracy values to z-scores
-n, --null-frequency <STRING>
background frequency file
-c, --minimum-score-cutoff <DOUBLE>
minimum z-score for per-residue prediction
-o, --outfile-prefix <STRING>
output file prefix for the three files generated: PREFIX_dat.txt, PREFIX_proftmb_pretty.txt PREFIX_proftmb_tabular.txt
-s, --static-model-data <STRING>
data representing the model architecture
-t, --trained-params <STRING>
params representing the encoded training data
-q, --test-blastQ-file-or-dir <STRING>
psiblast profile (-Q) or directory (full pathname or relative to current directory) with many profiles
-w, --single-sequence-name <STRING>
if -q option points to a single file, this is the name
-v, --list-blastQ-files <STRING>
list of psiblast files to process in directory (leave blank to process all files)
--outfile-pretty <STRING>
pretty output file (overrides automatic name PREFIX_proftmb_pretty.txt)
--outfile-tab
<STRING> tabulated output file (overrides automatic name PREFIX_proftmb_tabular.txt)
--outfile-dat
<STRING> data output file (overrides automatic name PREFIX_dat.txt)
--version
<BOOL> output version information and exit
--quiet
<BOOL> be quiet
EXAMPLES
proftmb @/usr/share/proftmb/options -q /usr/share/doc/proftmb/examples/example.Q -o /tmp/example
The above produces 3 output files: /tmp/example_proftmb_tabular.txt, /tmp/example_proftmb_pretty.txt and /tmp/example_dat.txt. Compare
these to the examples in /usr/share/doc/proftmb/examples.
BUGS
Please report bugs at <https://rostlab.org/bugzilla3/enter_bug.cgi?product=proftmb>.
COPYRIGHT
Copyright (C) 2004 Henry Bigelow
This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
This package is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MER-
CHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.
You should have received a copy of the GNU General Public License along with this program. If not, see <http://www.gnu.org/licenses/>.
proftmb 1.1.10 February 2012 PROFTMB(1)