fastq_masker(1) [debian man page]
FASTQ_MASKER(1) User Commands FASTQ_MASKER(1) NAME
fastq_masker - Masks nucleotides with 'N' (or other character) based on quality DESCRIPTION
usage: fastq_masker [-h] [-v] [-q N] [-r C] [-z] [-i INFILE] [-o OUTFILE] Part of FASTX Toolkit 0.0.13.2 by A. Gordon (gordon@cshl.edu) [-h] = This helpful help screen. [-q N] = Quality threshold - nucleotides with lower quality will be masked Default is 10. [-r C] = Replace low-quality nucleotides with character C. Default is 'N' [-z] = Compress output with GZIP. [-i INFILE] = FASTQ input file. default is STDIN. [-o OUTFILE] = FASTQ output file. default is STDOUT. [-v] = Verbose - report number of sequences. If [-o] is specified, report will be printed to STDOUT. If [-o] is not specified (and output goes to STDOUT), report will be printed to STDERR. SEE ALSO
The quality of this automatically generated manpage might be insufficient. It is suggested to visit http://hannonlab.cshl.edu/fastx_toolkit/commandline.html to get a better layout as well as an overview about connected FASTX tools. fastq_masker 0.0.13.2 May 2012 FASTQ_MASKER(1)
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FASTX_QUALITY_STATS(1) User Commands FASTX_QUALITY_STATS(1) NAME
fastx_quality_stats - FASTX Statistics DESCRIPTION
usage: fastx_quality_stats [-h] [-N] [-i INFILE] [-o OUTFILE] Part of FASTX Toolkit 0.0.13.2 by A. Gordon (gordon@cshl.edu) [-h] = This helpful help screen. [-i INFILE] = FASTQ input file. default is STDIN. [-o OUTFILE] = TEXT output file. default is STDOUT. [-N] = New output format (with more information per nucleotide/cycle). The *OLD* output TEXT file will have the following fields (one row per column): column = column number (1 to 36 for a 36-cycles read solexa file) count = number of bases found in this column. min = Lowest quality score value found in this column. max = Highest quality score value found in this column. sum = Sum of quality score values for this column. mean = Mean quality score value for this column. Q1 = 1st quartile quality score. med = Median quality score. Q3 = 3rd quartile quality score. IQR = Inter-Quartile range (Q3-Q1). lW = 'Left-Whisker' value (for boxplotting). rW = 'Right-Whisker' value (for boxplotting). A_Count = Count of 'A' nucleotides found in this column. C_Count = Count of 'C' nucleotides found in this column. G_Count = Count of 'G' nucleotides found in this column. T_Count = Count of 'T' nucleotides found in this column. N_Count = Count of 'N' nucleo- tides found in this column. max-count = max. number of bases (in all cycles) The *NEW* output format: cycle (previously called 'column') = cycle number max-count For each nucleotide in the cycle (ALL/A/C/G/T/N): count = number of bases found in this column. min = Lowest quality score value found in this column. max = Highest quality score value found in this column. sum = Sum of quality score values for this column. mean = Mean quality score value for this column. Q1 = 1st quartile quality score. med = Median quality score. Q3 = 3rd quartile quality score. IQR = Inter-Quartile range (Q3-Q1). lW = 'Left-Whisker' value (for boxplotting). rW = 'Right-Whisker' value (for boxplotting). SEE ALSO
The quality of this automatically generated manpage might be insufficient. It is suggested to visit http://hannonlab.cshl.edu/fastx_toolkit/commandline.html to get a better layout as well as an overview about connected FASTX tools. fastx_quality_stats 0.0.13.2 May 2012 FASTX_QUALITY_STATS(1)