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Top Forums Shell Programming and Scripting Script to search and extract the gene sub-location from gff file. Post 302534410 by reena2305 on Tuesday 28th of June 2011 12:24:15 AM
Old 06-28-2011
@panyam

sorry this was my first post, so I didn't have much idea.

Regarding problem:

Code:
gene            39389788..39395643

It is a particular gene position in the whole genome, now this gene is madeup of CDS, mRNA, Introns etc..the information is right below it like:

Code:
mRNA            join(39389788..39389839,39390696
 CDS             join(39390729..39390861,39391681..39..

etc..until the information of next gene comes..
like: (say gene2)
Code:
gene            39391466..39391614

So I have file with these 'gene' location, now I need to extract its sub-location, like whether its in CDS, mRNA or Intron(in case no match found).

The location of gene(that we need to find) is in separate file:

Code:
Gene_input_file: f3 
 sno_input_file: chr3  
 319 found_in_gene  52698648..52707224 at 52704105 and_count: 5457
 68 found_in_gene  52698648..52707224 at 52705463 and_count: 6815 
82 found_in_gene  52698648..52707224 at 52701967 and_count: 3319
 124 found_in_gene  39793218..40244467 at 40222682 and_count: 429464
 202 found_in_gene  9443305..10558922 at 10110734 and_count: 667429
 228 found_in_gene  46262602..46896241 at 46629723 and_count: 367121 ..and so on.

I have to read it line by line, extract gene position, then search it in the main gene info. (gff) file. like:

Code:
52698648..52707224 (of file2) match it in file1 and print its sub-location.

note: '..' denotes FROM postion 52698648 TO 52707224.

Last edited by reena2305; 06-28-2011 at 01:32 AM..
 

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SWISS::GNs(3pm) 					User Contributed Perl Documentation					   SWISS::GNs(3pm)

Name
       SWISS::GNs.pm

Description
       SWISS::GNs represents the GN lines within an SWISS-PROT + TrEMBL entry as specified in the user manual
	http://www.expasy.org/sprot/userman.html . The GNs object is a container object which holds a list of SWISS::GeneGroup objects.

Inherits from
       SWISS::ListBase.pm

Attributes
       "list"
	     Each list element is a SWISS::GeneGroup object.

       "and" (deprecated, for old format only)
	     Delimiter used between genes. Defaults to " AND ".

       "or" (deprecated, for old format only)
	     Delimiter used between gene names. Defaults to " OR ".

Methods
   Standard methods
       new
       fromText
       toText

   Reading/Writing methods
       text [($newText)]
	   Sets the text of the GN line to the parameter if it is present, and returns the (unwrapped) text of the line.  Also sets 'and' and 'or'
	   delimiters to the first occurrences of the words "OR" and "AND" in the line, conserving the case.

       lowercase (deprecated, for old format only)
	   Sets the GNs::and and GNs::or delimiters to their lower case values.

       uppercase (deprecated, for old format only)
	   Sets the GNs::and and GNs::or delimiters to their upper case values.

       getFirst()
	   Returns first gene name in gene line

       getTags($target)
	   Returns evidence tags associated with $target

	   $target is a string

       isPresent($target)
	   Returns 1 if $target is present in the GN line

	   $target is a string

       needsReCasing($target)
	   If $target is present in the GN line, but wrongly cased, method returns the matching name in its current case

	   $target is a string

       replace($newName, $target, $evidenceTag)
	   Replaces the first GN object in the GN line whose text attribute is $target with a new GN object whose text attribute is set to
	   $newName and whose evidenceTags attribute is is set using values set by splitting $evidenceTag on /, / (as name is not being changed,
	   programs should keep old tag and add new tag).  Does nothing if $target is not found.

       delete($target)
	   Removes synonym/single-member gene group matching $target. Note that if a "Name" is deleted, the first "Synonym" will be promoted to
	   "Name"

       addAsNewSynonym($newName, $target, $evidenceTag, $location)
	   Adds a new GN object (with text attribute set to new $newName, and evidenceTags attribute set to ($evidenceTag)), as a synonym to the
	   first gene group in which $target is a gene name.  Does nothing if $target is not found.  Will not add a duplicate gene name.
	   $location determines where in gene group new object is added: if $location == 1, 2, 3, ..., new object added in the 1st, 2nd, 3rd, ...
	   position; if $location == 0, new object added before $target; if $location == -1, new object added after $target (default); if
	   $location == -2, new object added at end of gene group.  Note that if the new synonym is inserted in the first postion, it will become
	   the "Name" and the previous "Name" will be downgraded to first "Synonym"

       addAsNewGeneGroup($newName, $target, $evidenceTag, $location)
	   Adds a new GeneGroup object, comprising 1 GN object (with text attribute set to new $newName, and evidenceTags attribute set to
	   ($evidenceTag)). Will not add a duplicate gene name.  $location and $target determine where in GNs line new group is added: if
	   $location == 1, 2, 3, ..., new object added in the 1st, 2nd, 3rd, ... position; if $location == 0, new object added before $target; if
	   $location == -1, new object added after $target (default); if $location == -2, new object added at end of GNs line.	Does nothing if
	   $target is not found, and $location == 0 or -1; otherwise $target does not need to be set.

       replaceGeneGroup($newGeneGroup, $target)
	   Replaces the first gene group containing $target with $newGeneGroup.  Creating the $newGeneGroup correctly is the user's responsibility

       getGeneGroup($target)
	   Returns the first gene group that contains $target

       setToOr()
	   Retruns a new GNs object, but with all GNs objects in a single gene group.  Needed when adding 'C' to 'A and B', when the relationship
	   of 'C' to 'A' and 'B' is unknown: the universal use of ' or ' is the default delimeter for TrEMBL entries

TRANSITION
The format of the GN line will change in 2004 from: GN (CYSA1 OR CYSA OR RV3117 OR MT3199 OR MTCY164.27) AND (CYSA2 OR GN RV0815C OR MT0837 OR MTV043.07C). to: GN Name=CysA1; Synonyms=CysA; OrderedLocusNames=Rv3117, MT3199; GN ORFNames=MtCY164.27; GN and GN Name=CysA2; OrderedLocusNames=Rv0815c, MT0837; ORFNames=MTV043.07c; This module supports both formats. To convert an entry from the old to the new format, do: $entry->GNs->is_old_format(0); perl v5.10.1 2006-01-26 SWISS::GNs(3pm)
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